RBM28 Antibody - BSA Free

Western Blot: RBM28 Antibody [NBP1-32908] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: RBM28 Antibody [NBP1-32908] - Paraformaldehyde-fixed HeLa, using RBM28 antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RBM28 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RBM28 Antibody - BSA Free (NBP1-32908) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human RBM28. The exact sequence is proprietary.
• Localization: Nucleus, nucleolus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBM28
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RBM28 Antibody - BSA Free

• FLJ10377
• RNA binding motif protein 28
• RNA-binding motif protein 28,2810480G15Rik
• RNA-binding protein 28

Background

The protein encoded by the RBM28 gene is a specific nucleolar component of the spliceosomal small nuclear ribonucleoprotein (snRNP)complexes . It specifically associates with U1, U2, U4, U5, and U6 small nuclear RNAs (snRNAs), possibly coordinating their transition through the nucleolus. Mutation in this gene causes alopecia, progressive neurological defects, and endocrinopathy (ANE syndrome), a pleiotropic and clinically heterogeneous disorder. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RBM28