RbBP5 Antibody - BSA Free

Western Blot: RbBP5 Antibody [NBP2-95234] - Analysis of extracts of various cell lines, using RbBP5 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.

Immunocytochemistry/Immunofluorescence: RbBP5 Antibody [NBP2-95234] - Analysis of A-549 cells using RbBP5 . Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: RbBP5 Antibody [NBP2-95234] - Rat Intestine using RBBP5 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: RbBP5 Antibody [NBP2-95234] - Human colon carcinoma using RBBP5 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: RbBP5 Antibody [NBP2-95234] - Mouse liver using RBBP5 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunoprecipitation: RbBP5 Antibody [NBP2-95234] - Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug RbBP5 antibody (NBP2-95234). Western blot was performed from the immunoprecipitate using RbBP5 antibody (NBP2-95234) at a dilution of 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RbBP5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RbBP5 Antibody - BSA Free (NBP2-95234) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 319-538 of human RBBP5 (NP_005048.2). SIWAQNQVENWSAFAPDFKELDENVEYEERESEFDIEDEDKSEPEQTGADAAEDEEVDVTSVDPIAAFCSSDEELEDSKALLYLPIAPEVEDPEENPYGPPPDAVQTSLMDEGASSEKKRQSSADGSQPPKKKPKTTNIELQGVPNDEVHPLLGVKGDGKSKKKQAGRPKGSKGKEKDSPFKPKLYKGDRGLPLEGSAKGKVQAELSQPLTAGGAISELL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBBP5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:500 - 1:1000
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RbBP5 Antibody - BSA Free

• RBQ3RBBP-5
• retinoblastoma binding protein 5
• Retinoblastoma-binding protein RBQ-3
• Set1c WD40 repeat protein, homolog
• SWD1

Background

RbBP5 is encoded by this gene is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBBP5