RB1 Antibody (RB1/7080R) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with RB1 antibody (RB1/7080R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu, Mu
• Applications: IHC
• Clone: RB1/7080R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

RB1 Antibody (RB1/7080R) - Azide and BSA Free Summary

• Description: Positive Controls: HeLa, A431 or BT-20 cells. Breast or Colon carcinoma.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant human full-length RB1 protein
• Epitope: aa 703-772
• Localization: Nucleus.
• Specificity: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product Its epitope is localized between aa 703-772 It shows no cross reaction with p107 or p130 It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells It does not stain the Rb-negative BT549 cells It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for RB1 Antibody (RB1/7080R) - Azide and BSA Free

• OSRC
• osteosarcoma
• p105-Rb
• pp110
• pRb
• RB
• RB1
• retinoblastoma 1
• retinoblastoma suspectibility protein
• retinoblastoma-associated protein

Background

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of ts function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RB1