RB1 Antibody (RB1/7080R) [DyLight 488]

• Reactivity: Hu, Mu
• Applications: IHC
• Clone: RB1/7080R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: DyLight 488

RB1 Antibody (RB1/7080R) [DyLight 488] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant human full-length RB1 protein
• Epitope: aa 703-772
• Localization: Nucleus.
• Specificity: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product Its epitope is localized between aa 703-772 It shows no cross reaction with p107 or p130 It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells It does not stain the Rb-negative BT549 cells It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for RB1 Antibody (RB1/7080R) [DyLight 488]

• OSRC
• osteosarcoma
• p105-Rb
• pp110
• pRb
• RB
• RB1
• retinoblastoma 1
• retinoblastoma suspectibility protein
• retinoblastoma-associated protein

Background

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of ts function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RB1