RB1 [p Ser780] Antibody (JE44-61)

Western Blot: RB1 [p Ser780] Antibody (JE44-61) [NBP3-32794] - Western blot analysis of RB1 on different lysates with Rabbit anti-RB1 antibody (NBP3-32794) at 1/1,000 dilution. Lane 1: Jurkat serum starved for 44 hours whole cell lysate Lane 2: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours whole cell lysate Lane 3: Jurkat serum starved for 44 hours then treated with pp for 1 hour whole cell lysate Lane 4: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours then treated with pp for 1 hour whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 106 kDa Observed band size: 106 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32794) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: JE44-61
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RB1 [p Ser780] Antibody (JE44-61) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic phosphopeptide corresponding to resudues surrounding Ser780 of human RB1 protein. (Uniprot: P06400)
• Modification: p Ser780
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 106 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RB1 [p Ser780] Antibody (JE44-61)

• OSRC
• osteosarcoma
• p105-Rb
• pp110
• pRb
• RB
• RB1
• retinoblastoma 1
• retinoblastoma suspectibility protein
• retinoblastoma-associated protein

Background

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of ts function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RB1