Rat CXCL2/CINC-3 DuoSet ELISA, 15 Plate

• Reactivity: Rt
• Applications: ELISA
• Conjugate: Biotin

Rat CXCL2/CINC-3 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Cxcl2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat CXCL2/CINC-3 DuoSet ELISA, 15 Plate

• chemokine (C-X-C motif) ligand 2
• CINC-2a
• CINC3
• CINC-3
• C-X-C Motif Chemokine 2
• CXCL2
• GRO beta
• GRO2 oncogene
• GRO2
• GROB
• Gro-beta
• Growth-regulated protein beta
• Macrophage inflammatory protein 2-alpha
• melanoma growth stimulatory activity beta
• MGSA beta
• MGSA-b
• MGSA-beta
• MIP2
• MIP-2
• MIP2A
• MIP-2a
• MIP2-alpha
• SCYB2

Background

CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.

Publications for CXCL2/GRO beta/MIP-2/CINC-3 (DY525)(10)

Publications using DY525

• Falter, J;Lohmeier, A;Eberl, P;Stoerr, EM;Koskimäki, J;Falter, L;Rossmann, J;Mederer, T;Schmidt, NO;Proescholdt, M; CXCR2-Blocking Has Context-Sensitive Effects on Rat Glioblastoma Cell Line Outgrowth (S635) in an Organotypic Rat Brain Slice Culture Depending on Microglia-Depletion (PLX5622) and Dexamethasone Treatment International journal of molecular sciences 2023-11-27 [PMID: 38069130] (Rat)
• HE Burzynski, VA Macht, JL Woodruff, JN Crawford, JM Erichsen, GG Piroli, CA Grillo, JR Fadel, LP Reagan Pyridostigmine bromide elicits progressive and chronic impairments in the cholinergic anti-inflammatory pathway in the prefrontal cortex and hippocampus of male rats Neurobiology of stress, 2022-04-15;18(0):100446. 2022-04-15 [PMID: 35573808] (Rat)
• MJ Jeong, S Jeon, HS Yu, WS Cho, S Lee, D Kang, Y Kim, YJ Kim, SY Kim Exposure to Nickel Oxide Nanoparticles Induces Acute and Chronic Inflammatory Responses in Rat Lungs and Perturbs the Lung Microbiome International Journal of Environmental Research and Public Health, 2022-01-04;19(1):. 2022-01-04 [PMID: 35010784] (Rat)
• WMT Kuwabara, CNF Yokota, R Curi, TC Alba-Loure Obesity and Type 2 Diabetes mellitus induce lipopolysaccharide tolerance in rat neutrophils Sci Rep, 2018-12-03;8(1):17534. 2018-12-03 [PMID: 30510205] (Rat)
• D Toumpanaki, V Vassilakop, I Sigala, P Zacharatos, I Vraila, V Karavana, S Theocharis, T Vassilakop The role of Src & ERK1/2 kinases in inspiratory resistive breathing induced acute lung injury and inflammation Respir. Res., 2017-12-13;18(1):209. 2017-12-13 [PMID: 29237457] (Rat)
• Vlaar AP, Hofstra JJ, Kulik W Supernatant of stored platelets causes lung inflammation and coagulopathy in a novel in vivo transfusion model. Blood, 2010-05-17;116(8):1360-8. 2010-05-17 [PMID: 20479286] (Rat)
• Hamacher J, Stammberger U, Roux J, Kumar S, Yang G, Xiong C, Schmid RA, Fakin RM, Chakraborty T, Hossain HM, Pittet JF, Wendel A, Black SM, Lucas R The lectin-like domain of tumor necrosis factor improves lung function after rat lung transplantation--potential role for a reduction in reactive oxygen species generation. Crit. Care Med., 2010-03-01;38(3):871-8. 2010-03-01 [PMID: 20081530] (Rat)
• Birrell MA, Catley MC, Hardaker E, Wong S, Willson TM, McCluskie K, Leonard T, Farrow SN, Collins JL, Haj-Yahia S, Belvisi MG Novel role for the liver X nuclear receptor in the suppression of lung inflammatory responses. J. Biol. Chem., 2007-08-31;282(44):31882-90. 2007-08-31 [PMID: 17766241] (Rat)
• Birrell MA, McCluskie K, Hardaker E, Knowles R, Belvisi MG Utility of exhaled nitric oxide as a noninvasive biomarker of lung inflammation in a disease model. Eur. Respir. J., 2006-09-27;28(6):1236-44. 2006-09-27 [PMID: 17005583] (Rat)
• Braun C, Hamacher J, Morel DR, Wendel A, Lucas R Dichotomal role of TNF in experimental pulmonary edema reabsorption. J. Immunol., 2005-09-01;175(5):3402-8. 2005-09-01 [PMID: 16116234] (Rat)

FAQs for CXCL2/GRO beta/MIP-2/CINC-3 (DY525). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Cxcl2