RASGRP 4 Antibody (1F3) - Azide and BSA Free

Immunohistochemistry-Paraffin: RASGRP 4 Antibody (1F3) [H00115727-M01] - Analysis of monoclonal antibody to RASGRP4 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.

Sandwich ELISA: RASGRP 4 Antibody (1F3) [H00115727-M01] - Detection limit for recombinant GST tagged RASGRP4 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RASGRP 4 Antibody (1F3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse RASGRP 4 Antibody (1F3) - Azide and BSA Free (H00115727-M01) is a monoclonal antibody validated for use in IHC, WB and ELISA. Anti-RASGRP 4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: RASGRP4 (NP_733748, 574 a.a. ~ 673 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CGLCCHKHCRDQVKVECKKRPGAKGDAGPPGAPVPSTPAPHASCGSEENHSYTLSLEPETGCQLRHAWTQTESPHPSWETDTVPCPVMDPPSTASSKLDS
• Specificity: RASGRP4 - RAS guanyl releasing protein 4
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RASGRP4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RASGRP 4 Antibody (1F3) - Azide and BSA Free

• guanyl nucleotide releasing protein 4
• RAS guanyl releasing protein 4
• RAS guanyl-releasing protein 4

Background

The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RASGRP 4 Antibody (H00115727-M01)(1)

Publication using H00115727-M01

• Shalom-Feuerstein R, Makovski V, Levy R et al. Galectin-3 regulates RasGRP4-mediated activation of N-Ras and H-Ras. Biochim Biophys Acta. 2008-03-25 [PMID: 18413234]

Bioinformatics

• Gene Symbol: RASGRP4
• Entrez:
  • 115727(Human)
• OMIM:
  • 607320(Human)