Biological Strategies: Western Blot: RAPGEF2 Antibody [NBP1-06549] - Detection of Human and Mouse PDZ-GEF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse ...read more
Immunohistochemistry: RAPGEF2 Antibody [NBP1-06549] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PDZ-GEF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for RAPGEF2 Antibody (NBP1-06549). (Showing 1 - 1 of 1 FAQs).
Hello - I'd be interested in using this antibody to detect endogenous rapgef2 in a rat cell line. We've detected this protein pharmacologically and by RT-PCR, so I strongly suspect that protein should be expressed as well. Was wondering (a) has this antibody every been used in rat tissue? and (b) in the right panel of the figure, Rapgef2 was co-IP'ed with another antibody - is this one available? (c) Do you have any idea what the bands at lower MW in the right image may correspond to?
In regards to your inquiries, A) The antibody has never been tested in rat samples as far as I am showing from the lab testing data. B) The other antibody that this one was Co-IP'ed with is one that is no longer available, which is why the catalog number is not on the datasheet. C) Our lab has not been able to characterize the lower molecular weight non-specific bands at this time.
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