RAPGEF2 Antibody - BSA Free

Western Blot: RAPGEF2 Antibody [NBP1-06549] - Whole cell lysate (50 ug) from HeLa, Hep-G2, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDZ-GEF1 antibodyused for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: RAPGEF2 Antibody [NBP1-06549] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PDZ-GEF1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: RAPGEF2 Antibody [NBP1-06549] - Detection of human PDZ-GEF1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PDZ-GEF1 antibody NBP1-06549 used for IP at 6 ug per reaction. For blotting immunoprecipitated PDZ-GEF1, NBP1-06549 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu, Ca
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RAPGEF2 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 975 and 1025 of human PDZ domain containing guanine nucleotide exchange factor (GEF) 1 (NP_055062.1).
• Predicted Species: Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAPGEF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:1000 - 1:5000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 or Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RAPGEF2 Antibody - BSA Free

• CNrasGEF
• KIAA0313
• Neural RAP guanine nucleotide exchange protein
• nRap GEP
• NRAPGEP
• PDZ domain containing guanine nucleotide exchange factor (GEF) 1
• PDZGEF1
• PDZ-GEF1PDZ domain-containing guanine nucleotide exchange factor 1
• RA(Ras/Rap1A-associating)-GEF
• RA-GEFDKFZP586O1422
• Rap guanine nucleotide exchange factor (GEF) 2
• rap guanine nucleotide exchange factor 2
• Rap-GEP

Background

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAPGEF2 Antibody (NBP1-06549)(3)

Publications using NBP1-06549

• Li Y, Dillon TJ, Takahashi M et al. PKA-independent Ras activation cooperates with Rap1 to mediate activation of ERKs by cAMP. J. Biol. Chem. 2016-08-16 [PMID: 27531745] (WB, Human)
• Zhang H, Nie W, Zhang X et al. NEDD4-1 Regulates Migration and Invasion of Glioma Cells through CNrasGEF Ubiquitination In Vitro. PLoS One 2013-12-10 [PMID: 24340059] (WB, Human)
• Emery AC, Eiden MV, Mustafa T, Eiden LE. Rapgef2 Connects GPCR-Mediated cAMP Signals to ERK Activation in Neuronal and Endocrine Cells. Sci Signal 2013-06-25 [PMID: 23800469]

FAQs for RAPGEF2 Antibody (NBP1-06549). (Showing 1 - 1 of 1 FAQs).

1. Hello - I'd be interested in using this antibody to detect endogenous rapgef2 in a rat cell line. We've detected this protein pharmacologically and by RT-PCR, so I strongly suspect that protein should be expressed as well. Was wondering (a) has this antibody every been used in rat tissue? and (b) in the right panel of the figure, Rapgef2 was co-IP'ed with another antibody - is this one available? (c) Do you have any idea what the bands at lower MW in the right image may correspond to?
   • In regards to your inquiries, A) The antibody has never been tested in rat samples as far as I am showing from the lab testing data. B) The other antibody that this one was Co-IP'ed with is one that is no longer available, which is why the catalog number is not on the datasheet. C) Our lab has not been able to characterize the lower molecular weight non-specific bands at this time.

Bioinformatics

• Gene Symbol: RAPGEF2
• Entrez:
  • 9693(Human)
• Uniprot:
  • Q9Y4G8(Human)