RANK/TNFRSF11A Antibody (9A725) [DyLight 488]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, Flow-CS, IHC, IHC-P
Clone
9A725
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

RANK/TNFRSF11A Antibody (9A725) [DyLight 488] Summary

Immunogen
A fusion protein containing amino acid residues 326-616 of human RANK was used as immunogen. This antibody recognizes an epitope located between amino acid residues 330-427 of human RANK (NP_003830).
Specificity
In RAW cells, a 97 kDa is observed.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF11A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Flow (Cell Surface)
Application Notes
Bharti et al (2004) and Reigel et al (2012) both published the RANK antibody clone 9A725 for Flow (Cell Surface). However, the epitope range recognized by this antibody is a potential cytoplasmic region as defined by www.uniprot.org. Hence, researchers are encouraged to refer to both the publication and the bioinformatics data bases for additional information in order to make their own determination regarding the suitability of the antibody for cell surface flow cytometry.

Reactivity Notes

Cross reacts with Human and Mouse. Rat reactivity reported in scientific literature (PMID: 24376119). Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for RANK/TNFRSF11A Antibody (9A725) [DyLight 488]

  • CD265 antigen
  • CD265
  • FEO
  • loss of heterozygosity, 18, chromosomal region 1
  • ODFR
  • ODFROSTS
  • OFE
  • OPTB7
  • Osteoclast differentiation factor receptor
  • PDB2
  • RANK
  • RANKLOH18CR1
  • Receptor activator of NF-KB
  • receptor activator of nuclear factor-kappa B
  • TNFRSF11A
  • TRANCE R
  • TRANCER
  • tumor necrosis factor receptor superfamily member 11A
  • tumor necrosis factor receptor superfamily, member 11a, activator of NFKB
  • tumor necrosis factor receptor superfamily, member 11a, NFKB activator

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-56508APC
Biotin Labeled NB100-56508B
DyLight 350 Labeled NB100-56508UV
DyLight 405 Labeled NB100-56508V
DyLight 550 Labeled NB100-56508R
DyLight 650 Labeled NB100-56508C
DyLight 680 Labeled NB100-56508FR
DyLight 755 Labeled NB100-56508IR
HRP Labeled NB100-56508H
PE Labeled NB100-56029
PerCP Labeled NB100-56508PCP

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Bioinformatics

Gene Symbol TNFRSF11A
Entrez