Ran Antibody

Images

 
Western Blot: Ran Antibody [NBP1-31776] - Mouse tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAN antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Ran Antibody [NBP1-31776] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: Ran Antibody [NBP1-31776] - Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, Dr, Mk, Sh, Xp, ZeSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.6 mg/ml

Order Details

Ran Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RAN. The exact sequence is proprietary.
Localization
Nucleus, Cytoplasm, Melanosome |
Predicted Species
Rat (100%), Bovine (100%), Drosophila (95%), Canine (100%), Chicken (100%), Monkey (100%), Xenopus (100%), Sheep (100%), Zebrafish (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-31776 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (100%) and Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.6 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ran Antibody

  • Androgen receptor-associated protein 24
  • ARA24Gsp1
  • GTPase Ran
  • GTP-binding nuclear protein Ran
  • guanosine triphosphatase Ran
  • member RAS oncogene family
  • OK/SW-cl.81
  • RAN, member RAS oncogene family
  • RanGTPase
  • Ras-like protein TC4
  • Ras-related nuclear protein
  • TC4

Background

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-85637
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-79814
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NBP1-82520
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-74480
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB400-148
Species: Hu, Mu, Rt, Po, ChHa, Pm
Applications: WB, EM, ICC/IF, IHC, IHC-P
NB100-79802
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP, PLA
NBP2-02623
Species: Hu, Rt, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-85727
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF6260
Species: Hu
Applications: WB
NBP1-52556
Species: Hu, Mu, Po, Bt, Bv, Ca, Mk, Pm, Rb
Applications: IHC-P
NBP1-83213
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-15087
Species: Hu, Mu
Applications: WB
MAB1259
Species: Hu
Applications: CyTOF-reported, ICC, ICFlow
NB100-56603
Species: Hu, Pm
Applications: WB, IHC, IHC-P
NBP1-47602
Species: Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP2-01462
Species: Hu
Applications: WB, Flow, ICC/IF

Publications for Ran Antibody (NBP1-31776) (0)

There are no publications for Ran Antibody (NBP1-31776).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for Ran Antibody (NBP1-31776) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-31776:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Ran NBP1-31776
Enlarge
reviewed by:
Ye Zhang
WB Human 04/13/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman melanoma cells, Sample Amount: 25ug
SpeciesHuman
Lot40128

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ran Antibody (NBP1-31776) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Ran Products

Bioinformatics Tool for Ran Antibody (NBP1-31776)

Discover related pathways, diseases and genes to Ran Antibody (NBP1-31776). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Ran Antibody (NBP1-31776)

Discover more about diseases related to Ran Antibody (NBP1-31776).
 

Pathways for Ran Antibody (NBP1-31776)

View related products by pathway.

PTMs for Ran Antibody (NBP1-31776)

Learn more about PTMs related to Ran Antibody (NBP1-31776).
 

Research Areas for Ran Antibody (NBP1-31776)

Find related products by research area.

Blogs on Ran.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Ye Zhang
04/13/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol RAN