RalA Antibody (JE54-12)

Western Blot: RalA Antibody (JE54-12) [NBP3-32891] - Western blot analysis of RalA on different lysates with Rabbit anti-RalA antibody (NBP3-32891) at 1/500 dilution. Lane 1: LOVO cell lysate (10 ug/Lane) Lane 2: HCT116 cell lysate (10 ug/Lane) Lane 3: HT-29 cell lysate (10 ug/Lane) Lane 4: SW480 cell lysate (10 ug/Lane) Lane 5: 293 cell lysate (10 ug/Lane) Lane 6: Human kidney tissue lysate (20 ug/Lane) Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32891) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: RalA Antibody (JE54-12) [NBP3-32891] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-RalA antibody (NBP3-32891) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32891) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: RalA Antibody (JE54-12) [NBP3-32891] - Flow cytometric analysis of HUVEC cells labeling RalA. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32891, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clone: JE54-12
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RalA Antibody (JE54-12) Summary

• Description: Novus Biologicals Rabbit RalA Antibody (JE54-12) (NBP3-32891) is a recombinant monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human RalA aa 107-206 / 206. (Uniprot: P11233)
• Localization: Cell membrane, Mitochondrion.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RALA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:500-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RalA Antibody (JE54-12)

• HINCONS
• RAL
• RalA
• Rasl1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RALA