RAIDD/CRADD Antibody (1F8) - Azide and BSA Free

Western Blot: RAIDD/CRADD Antibody (1F8) [H00008738-M01] - CRADD monoclonal antibody (M01), clone 1F8 Analysis of CRADD expression in K-562.

Immunocytochemistry/Immunofluorescence: RAIDD/CRADD Antibody (1F8) [H00008738-M01] - Analysis of monoclonal antibody to CRADD on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: RAIDD/CRADD Antibody (1F8) [H00008738-M01] - Analysis of CRADD expression in transfected 293T cell line by CRADD monoclonal antibody (M01), clone 1F8.Lane 1: CRADD transfected lysate(23 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: RAIDD/CRADD Antibody (1F8) [H00008738-M01] - Detection limit for recombinant GST tagged CRADD is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 1F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RAIDD/CRADD Antibody (1F8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CRADD (AAH37905, 1 a.a. ~ 199 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEARDKQVLRSLRLELGAEVLVEGLVLQYVYEEGILTENHIQEINAQTTGLRKTMLLLDILPSRGPKAFDTFLDSLQEFPWVREKLKKAREEAMTDLPAGDRLTGIPSHILNSSPSDRQINQLAQRLGPEWEPMVLSLGLSQTDIYRCKANHPHNVQSQVVEAFIRWRQRFGKQATFQSLHNGLRAVEVDPSLLLHMLE
• Specificity: CRADD - CASP2 and RIPK1 domain containing adaptor with death domain
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRADD
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RAIDD/CRADD Antibody (1F8) - Azide and BSA Free

• CASP2 and RIPK1 domain containing adaptor with death domain
• Caspase and RIP adapter with death domain
• caspase and RIP adaptor with death domain
• CRADD
• death domain containing protein CRADD
• death domain-containing protein CRADD
• MGC9163
• RAIDD
• RAIDDdeath adaptor molecule RAIDD
• RIP-associated ICH1/CED3-homologous protein with death domain
• RIP-associated protein with a death domain

Background

The protein encoded by this gene is a death domain (CARD/DD)-containing protein and has been shown to induce cell apoptosis. Through its CARD domain, this protein interacts with, and thus recruits, caspase 2/ICH1 to the cell death signal transduction complex that includes tumor necrosis factor receptor 1 (TNFR1A), RIPK1/RIP kinase, and numbers of other CARD domain-containing proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAIDD/CRADD Antibody (H00008738-M01)(1)

Publication using H00008738-M01

• Di Donato N, Jean YY, Maga AM et al. Mutations in CRADD Result in Reduced Caspase-2-Mediated Neuronal Apoptosis and Cause Megalencephaly with a Rare Lissencephaly Variant. Am J Hum Genet 2016-11-03 [PMID: 27773430]

Bioinformatics

• Gene Symbol: CRADD
• Entrez:
  • 8738(Human)
• OMIM:
  • 603454(Human)
• Uniprot:
  • AAH37905(Human)