Radixin Antibody

Western Blot: Radixin Antibody [NB100-41100] - (0.03ug/ml) Analysis of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Radixin Antibody Summary

• Description: Novus Biologicals Goat Radixin Antibody (NB100-41100) is a polyclonal antibody validated for use in WB and ELISA. Anti-Radixin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QARDETKKTQND corresponding to internal region according to NP_002897.1.
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RDX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 80 kDa band observed in human lymph node, tonsil and spleen lysates is reported in scientific literature (PMID: 7592691).
• Control:

  Radixin Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Radixin Antibody

• deafness, autosomal recessive 24
• DFNB24
• radixin

Background

Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Radixin Antibody (NB100-41100)(1)

Publication using NB100-41100

• Ivetic A, Florey O, Deka J et al. Mutagenesis of the ezrin-radixin-moesin binding domain of L-selectin tail affects shedding, microvillar positioning, and leukocyte tethering. J Biol Chem 2004-08-06 [PMID: 15178693]

Bioinformatics

• Gene Symbol: RDX
• Entrez:
  • 5962(Human)
  • 19684(Mouse)
  • 315655(Rat)
• Uniprot:
  • NP_002897.1(Human)