Rad50 Overexpression Lysate (Native)

Images

 
Western Blot: RAD50 Overexpression Lysate (Adult Normal) [NBL1-15116] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAD50.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Rad50 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC211751 and based on accession number NM_005732. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens RAD50 homolog (S. cerevisiae) (RAD50), transcript variant 1, mRNA.
Gene
RAD50

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
153.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Rad50

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Rad50 Overexpression Lysate (Native)

  • DNA repair protein RAD50
  • EC 3.6
  • EC 3.6.1.15
  • EC 3.6.3
  • EC 3.6.3.27
  • hRad50
  • NBSLD
  • RAD50 (S. cerevisiae) homolog
  • RAD50 homolog (S. cerevisiae)
  • Rad50
  • RAD502
  • RAD50-2

Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq]. Transcript Variant: This variant (1) contains an internal region absent in variant 2. The resulting protein is 139 aa longer at its N-terminus, as compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Rad50 Lysate (NBL1-15116) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Blogs on Rad50.

RAD50 and DNA Damage Response
DNA repair protein RAD50 is a component of the MRN complex (Mre11-RAD50-Nbs1) responsible for DNA double strand break (DSB) repair. DSBs are caused by ionizing radiation, certain chemotherapy drugs, metabolic reactive oxygen species (ROS), replication...  Read full blog post.

The MRE11 Complex and DNA Damage Response
The maintenance of genome stability depends on the DNA damage response (DDR) which is a complex signaling network including cell cycle checkpoints, DNA repair and damage tolerance pathways. The DDR complex has the ability to sense DNA damage and tra...  Read full blog post.

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Bioinformatics

Gene Symbol RAD50
Entrez