Rad50 Antibody

Images

 
Western Blot: RAD50 Antibody [NB100-1488] - Detection of Human Rad50 on HeLa whole cell lysate using NB100-1488.
Immunohistochemistry-Paraffin: RAD50 Antibody [NB100-1488] - FFPE section of mouse squamous cell carcinoma. Affinity purified rabbit anti-Rad50 used at a dilution of 1:250. Detection DAB staining using anti-Rabbit IHC ...read more
Immunohistochemistry-Paraffin: RAD50 Antibody [NB100-1488] - FFPE section of human larynx squamous cell carcinoma. Affinity purified rabbit anti-Rad50 used at a dilution of 1:250. Detection with DAB staining using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Rad50 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 700 and 750 of human Rad50 using the numbering given in entry NP_005723.2 (GeneID 10111).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAD50
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:500-1:2000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
Rad50 Lysate (NBL1-15116)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Rad50 Antibody

  • DNA repair protein RAD50
  • EC 3.6
  • EC 3.6.1.15
  • EC 3.6.3
  • EC 3.6.3.27
  • hRad50
  • NBSLD
  • RAD50 (S. cerevisiae) homolog
  • RAD50 homolog (S. cerevisiae)
  • Rad50
  • RAD502
  • RAD50-2

Background

Rad50, a 153kDa protein involved in DNA double strand break repair, is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Rad50 Antibody (NB100-1488) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Rad50 Products

Bioinformatics Tool for Rad50 Antibody (NB100-1488)

Discover related pathways, diseases and genes to Rad50 Antibody (NB100-1488). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

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Research Areas for Rad50 Antibody (NB100-1488)

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Blogs on Rad50.

RAD50 and DNA Damage Response
DNA repair protein RAD50 is a component of the MRN complex (Mre11-RAD50-Nbs1) responsible for DNA double strand break (DSB) repair. DSBs are caused by ionizing radiation, certain chemotherapy drugs, metabolic reactive oxygen species (ROS), replication...  Read full blog post.

The MRE11 Complex and DNA Damage Response
The maintenance of genome stability depends on the DNA damage response (DDR) which is a complex signaling network including cell cycle checkpoints, DNA repair and damage tolerance pathways. The DDR complex has the ability to sense DNA damage and tra...  Read full blog post.

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Bioinformatics

Gene Symbol RAD50
Entrez
Uniprot