RAD18 Antibody

Western Blot: RAD18 Antibody [NB100-61063] - Detection of Human RAD18 on HeLa whole cell lysate using NB100-61063. RAD18 was also immunoprecipitated by rabbit anti-RAD18 antibody NB100-61062.

• Reactivity: Hu
• Applications: WB, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RAD18 Antibody Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit RAD18 Antibody (NB100-61063) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-RAD18 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 445 and 495 of human E3 ubiquitin-protein ligase RAD18 using the numbering given in entry NP_064550.2 (GeneID 56852).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAD18
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockout Validated
  • Western Blot 1:2000-1:10000
• Application Notes: KO Validation was reported in scientific literature (PMID: 27146073).
• Control:

  RAD18 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RAD18 Antibody

• EC 6.3.2.-
• hHR18
• hRAD18
• postreplication repair protein hRAD18p
• Postreplication repair protein RAD18
• RAD18 homolog (S. cerevisiae)
• RAD18, S. cerevisiae, homolog
• RING finger protein 73
• RNF73E3 ubiquitin-protein ligase RAD18

Background

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAD18 Antibody (NB100-61063)(4)

Publications using NB100-61063

• Wells JP, Chang EY, Dinatto L et al. RAD18 opposes transcription-associated genome instability through FANCD2 recruitment PLoS genetics 2022-12-01 [PMID: 36480547] (ICC/IF, Human)
  
  Details:
  1:1000 ICC/IF
• Barnes RP, Tsao WC, Moldovan GL, Eckert kA. DNA Polymerase Eta Prevents Tumor Cell Cycle Arrest And Cell Death During Recovery from Replication Stress. Cancer Res. 2018-10-08 [PMID: 30297532] (WB, Human)
• Choe KN, Nicolae CM, Constantin D et al. HUWE1 interacts with PCNA to alleviate replication stress. EMBO Rep. 2016-05-04 [PMID: 27146073] (IP, WB, Human)
• Williams SA, Longerich S, Sung P et al. The E3 ubiquitin ligase RAD18 regulates ubiquitylation and chromatin loading of FANCD2 and FANCI. Blood 2011-05-01 [PMID: 21355096]

Bioinformatics

• Gene Symbol: RAD18
• Entrez:
  • 56852(Human)
• Uniprot:
  • Q9NS91(Human)