![Western Blot: Rab7a Antibody [NBP2-24591] - Analysis of human Rab7 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse NIH 3T3 and 4) RAW using Rab7 at 0.25 ug/ml.](http://images.novusbio.com/fullsize/Rab7a-Antibody-Western-Blot-NBP2-24591-img0004.jpg "Western Blot: Rab7a Antibody [NBP2-24591] - Analysis of human Rab7 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse NIH 3T3 and 4) RAW using Rab7 at 0.25 ug/ml.")
| Reactivity | Hu, Mu, Rt, Bv, Ca, Fi, Pm, RbSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit Rab7a Antibody - BSA Free (NBP2-24591) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Rab7a Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | A portion of amino acids 90-140 of human Rab7 protein was used as the immunogen for this antibody. |
| Marker | Late Endosome Marker |
| Predicted Species | Rat (98%), Canine (98%), Primate (98%), Bovine (98%), Rabbit (96%), Fish (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RAB7A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NBP2-24591 | Applications | Species |
|---|---|---|
| Goswami B, Ahuja D, Pastr� D, Ray PS. p53 and HuR combinatorially control the biphasic dynamics of microRNA-125b in response to genotoxic stress Communications Biology 2023-01-27 [PMID: 36707647] (Western Blot) | Western Blot |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Teddy Chan |
WB | Human | 02/08/2019 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for Rab7a Antibody (NBP2-24591)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP2-24591] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Rab7A at 2ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Rab7a-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24591-img0006.jpg "Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP2-24591] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Rab7A at 2ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: Rab7a Antibody [NBP2-24591] - Analysis of human skeletal muscle using RAB7A antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Rab7a-Antibody-Immunohistochemistry-Paraffin-NBP2-24591-img0003.jpg "Immunohistochemistry-Paraffin: Rab7a Antibody [NBP2-24591] - Analysis of human skeletal muscle using RAB7A antibody at 10 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP2-24591] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Rab7A at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Rab7a-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24591-img0005.jpg "Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP2-24591] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Rab7A at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
