Rab7a Antibody (5A10A4)

Western Blot: Rab7a Antibody (4X3P6) [NBP3-15444] - Western blot analysis of extracts of various cell lines, using Rab7a Rabbit mAb (NBP3-15444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western Blot: Rab7a Antibody (5A10A4) [Rab7a] - Western blot analysis of various lysates using Rab7a Rabbit mAb at 1:55000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.

Immunocytochemistry/ Immunofluorescence: Rab7a Antibody (5A10A4) [Rab7a] - Confocal imaging of NIH/3T3 cells using Rab7a Rabbit mAb followed by a further incubation with ABflo 488-conjugated Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with Cy3 Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of NIH/3T3 cells using RAB7 Rabbit mAb (dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

RAB7 from 300 µg extracts of HeLa cells was performed using 0.5 µg of RAB7 Rabbit mAb. Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1x Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using RAB7 Rabbit mAb at a dilution of 1:5000.

Analysis of paraffin-embedded Mouse testis tissue using RAB7 Rabbit mAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 5A10A4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Rab7a Antibody (5A10A4) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 108-207 of human Rab7a (NP_004628.4). IQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAKASAESCSC
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RAB7A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL.
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:8000
  • Immunohistochemistry-Paraffin 1:1000 - 1:4000
  • Immunoprecipitation 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • Western Blot 1:55000 - 1:220000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Rab7a Antibody (5A10A4)

• CMT2B
• FLJ20819
• member RAS oncogene family
• PRO2706
• PSN
• RAB7
• Rab7a
• RAB7A, member RAS oncogene family
• Ras-associated protein RAB7
• ras-related protein Rab-7a

Background

RAB7 is a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAB7A