RAB6A Antibody (HL1047) - Azide and BSA Free

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701] - Wild-type (WT) and RAB6A knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (NBP3-13701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: RAB6A Antibody (HL1047) [NBP3-13701] - RAB6A antibody [HL1047] detects RAB6A protein at Golgi apparatus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. RAB6A stained by RAB6A antibody [HL1047] (NBP3-13701) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (NBP3-13701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: RAB6A Antibody (HL1047) [NBP3-13701] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (NBP3-13701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western blot shows lysates of HAP1 cell line and RAB6A knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with RAB6A Antibody (HL1047) (Catalog # NBP3-13701) followed by HRP-conjugated secondary antibody. A specific band was detected for RAB6A at approximately 24 kDa (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. Primary antibody dilution used: 1/200. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (NBP3-13701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody [HL1047] (NBP3-13701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Dr, Ze
• Applications: WB, ICC/IF, IHC, KO
• Clone: HL1047
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RAB6A Antibody (HL1047) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Full length human RAB6A recombinant protein.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RAB6A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Knockout Validated Validated for Knockout from YCharOS Inc. (ycharos.com)
  • Western Blot 1:500-1:3000
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for RAB6A Antibody (HL1047) - Azide and BSA Free

• GTP-binding protein RAB6A'
• GTP-binding protein RAB6B
• Oncogene RAB6
• Rab GTPase
• Rab-6
• RAB6, member RAS oncogene family
• Rab6A variant 3
• RAB6A'
• RAB6A, member RAS oncogene family
• RAB6B
• RAB6rab-6
• ras-related protein Rab-6A

Background

Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAB6A