Rab4 Antibody (SR27-08)

Western Blot: Rab4 Antibody (SR27-08) [NBP2-67317] - Western blot analysis of Rab4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb

Immunocytochemistry/Immunofluorescence: Rab4 Antibody (SR27-08) [NBP2-67317] - Staining Rab4A in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Western Blot: Rab4 Antibody (SR27-08) [NBP2-67317] - Analysis of Rab4A on different cell lysates using anti-Rab4A antibody at 1/500 dilution. Positive control: Lane 1: MCF-7 Lane 2: 293T

Product Discontinued

Rab4 Antibody (SR27-08) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Rab4 aa 169-218 / 218. (SwissProt: P20338 Human; SwissProt: P56371 Mouse; SwissProt: P05714 Rat)
• Localization: Cytoplasm, Membrane
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RAB4A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Rab4 Antibody (SR27-08)

• Oncogene RAB4
• RAB4, member RAS oncogene family
• RAB4A, member RAS oncogene family
• RAB4HRES-1/RAB4
• ras-related protein Rab-4A

Background

Rab4 is a small GTPase that regulates recycling of proteins, such as GPCRs, from the early endosomes to the cell surface. Overexpression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. Rab4 is also involved in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin. Association of Rab4 with transferrin receptor containing early endosomes is mediated through the geranylgeranyl groups at its carboxyl terminus. Rab4 can be used as a marker for early endosomes (see Daro et al. 1996 and Zuk et al. 1999 for more information). Membrane association is also cell cycle dependent, as phosphorylation at its C-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAB4A