Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody

Western blot shows lysates of SW13 human adrenal cortex adenocarcinoma cell line and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pyruvate Dehydrogenase Phosphatase/PDP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7260) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pyruvate Dehydrogenase Phosphatase/PDP1 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody Summary

• Immunogen: E. coli-derived recombinant human Pyruvate Dehydrogenase Phosphatase/PDP1
  Ala72-Glu537
  Accession # Q9P0J1
• Specificity: Detects human Pyruvate Dehydrogenase Phosphatase/PDP1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PDP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody

• [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
• EC 3.1.3
• EC 3.1.3.43
• MGC119646
• PDH
• PDP 1
• PDP1
• PDPC 1
• PDPC
• PDPFLJ32517
• PPM2C
• PPM2CFLJ56179
• Protein phosphatase 2C
• protein phosphatase 2C, magnesium-dependent, catalytic subunit
• pyruvate dehydrogenase (Lipoamide) phosphatase-phosphatase
• Pyruvate dehydrogenase phosphatase catalytic subunit 1
• Pyruvate Dehydrogenase Phosphatase
• pyruvate dehyrogenase phosphatase catalytic subunit 1

Background

PPM2C (Protein phosphatase mitochondrial 2C; also PDP1) is a 52-54 kDa member of the PP2C family of enzymes. It is expressed in skeletal muscle and adipocytes, and represents one of two subunits that comprise PDP (pyruvate dehydrogenase phosphatase). PPMC2 is found within the mitochondrial matrix space where, as part of PDP, it dephosphorylates the E1 component of the pyruvate dehydrogenase complex, resulting in its activation, and the downstream generation of acetyl-CoA and NADH from pyruvate. Human PPM2C is 537 amino acids (aa) in length. It contains a mitochondrion targeting sequence (aa 1-71), followed by a mature region that possesses a catalytic domain between aa 109-440. There are three potential isoform variants. Two contain alternative start sites 25 and 59 aa upstream of the standard start site, respectively. A third shows a 12 aa substitution for aa 105-116. Over aa 72-537, human PPM2C shares 99% aa identity with mouse PPM2C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody (AF7260)(1)

Publication using AF7260

• HE Farrell, K Bruce, C Lawler, M Oliveira, R Cardin, N Davis-Poyn, PG Stevenson Murine Cytomegalovirus Spreads by Dendritic Cell Recirculation MBio, 2017-10-03;8(5):. 2017-10-03 [PMID: 28974616] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: PDP1
• Uniprot:
  • Q9P0J1(Human)