Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (SR1677) - BSA Free

Western Blot-NBP3-22675-Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] -Antibody (SR1677)-Analysis of Phospho-PDHA1 (S293) expression in 293T cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clone: SR1677
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (SR1677) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human phospho-Pyruvate Dehydrogenase E1-alpha subunit [Ser293] (Uniprot #: P08559)
• Modification: p Ser293
• Specificity: Detects endogenous levels of total phospho-Pyruvate Dehydrogenase E1-alpha subunit [Ser293]
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PDHA1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (SR1677) - BSA Free

• EC 1.2.4.1
• PDHA
• PDHE1-A type I
• PHE1APDHCE1A
• pyruvate dehydrogenase (lipoamide) alpha 1
• pyruvate dehydrogenase complex, E1-alpha polypeptide 1
• pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Background

Pyruvate Dehydrogenase E1-alpha subunit (PDHE1 alpha) is a member of the pyruvate dehydrogenase complex (PDC) and is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1 or PDHE1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex. Enhanced expression of pyruvate dehydrogenase kinase-1 (PDK-1) results in phosphorylation of PDHE1 alpha at Serine 293, which in turn produces nearly complete inhibition of the pyruvate dehydrogenase complex (PDC). A recent study shows that inhibition of PDC activity in cancer cells restores normoxic stablization of HIF-1 alpha by glycolytic metabolites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDHA1