Pyruvate Carboxylase Antibody (SR1128) - BSA Free

Western Blot: Pyruvate Carboxylase Antibody (SR1128) [NBP3-22020] - Western blot analysis using NBP3-22020 in HepG2 cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: SR1128
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Pyruvate Carboxylase Antibody (SR1128) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human Pyruvate Carboxylase (Uniprot #: P11498)
• Specificity: Detects endogenous levels of total Pyruvate Carboxylase
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PC
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Pyruvate Carboxylase Antibody (SR1128) - BSA Free

• EC 6.4.1
• EC 6.4.1.1
• PCBPyruvic carboxylase
• Pyruvate Carboxylase
• pyruvate carboxylase, mitochondrial

Background

PCB encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PC