PYCRL Antibody (OTI1B12)

Western Blot: PYCRL Antibody (OTI1B12) [NBP2-03337] - Analysis of extracts (35ug) from 9 different cell lines by using anti-PYCRL monoclonal antibody.

Immunocytochemistry/Immunofluorescence: PYCRL Antibody (OTI1B12) [NBP2-03337] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCRL.

Immunohistochemistry-Paraffin: PYCRL Antibody (OTI1B12) [NBP2-03337] - Staining of paraffin-embedded Human thyroid tissue using anti-PYCRL mouse monoclonal antibody.

Flow Cytometry: PYCRL Antibody (OTI1B12) [NBP2-03337] - HEK293T cells transfected with either PYCRL Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCRL antibody , and then analyzed by flow cytometry.

Western Blot: PYCRL Antibody (OTI1B12) [NBP2-03337] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCRL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCRL.

Immunohistochemistry-Paraffin: PYCRL Antibody (OTI1B12) [NBP2-03337] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PYCRL mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PYCRL Antibody (OTI1B12) [NBP2-03337] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PYCRL mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PYCRL Antibody (OTI1B12) [NBP2-03337] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PYCRL mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PYCRL Antibody (OTI1B12) [NBP2-03337] - Staining of paraffin-embedded Human Kidney tissue using anti-PYCRL mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PYCRL Antibody (OTI1B12) [NBP2-03337] - Staining of paraffin-embedded Human pancreas tissue using anti-PYCRL mouse monoclonal antibody.

• Reactivity: Hu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.85 mg/ml

PYCRL Antibody (OTI1B12) Summary

• Immunogen: Full length human recombinant protein of human PYCRL (NP_075566) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PYCR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 28.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.85 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PYCRL Antibody (OTI1B12)

• EC 1.5.1.2
• FLJ13852
• P5C reductase 3
• P5CR 3
• pyrroline-5-carboxylate reductase 3
• Pyrroline-5-carboxylate reductase-like protein
• pyrroline-5-carboxylate reductase-like

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PYCR3
• Entrez:
  • 65263(Human)