PYCR2 Antibody - Azide and BSA Free

Western Blot: PYCR2 Antibody - Azide and BSA Free [PYCR2] - Western blot analysis of various lysates, using PYCR2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.

Immunocytochemistry/Immunofluorescence: PYCR2 Antibody [NBP2-93882] - Analysis of C6 cells using PYCR2 at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: PYCR2 Antibody [NBP2-93882] - Human liver cancer using PYCR2 Rabbit pAb at dilution of 1:100 (40x lens).

Immunocytochemistry/Immunofluorescence: PYCR2 Antibody [NBP2-93882] - Analysis of HeLa cells using PYCR2 at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: PYCR2 Antibody [NBP2-93882] - Analysis of L929 cells using PYCR2 at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: PYCR2 Antibody [NBP2-93882] - Mouse kidney using PYCR2 Rabbit pAb at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: PYCR2 Antibody [NBP2-93882] - Rat liver using PYCR2 Rabbit pAb at dilution of 1:100 (40x lens).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PYCR2 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 271-320 of human PYCR2 (NP_037460.2). MADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PYCR2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for PYCR2 Antibody - Azide and BSA Free

• EC 1.5.1.2
• FLJ54750
• P5C reductase 2
• P5CR 2
• P5CR2
• pyrroline 5-carboxylate reductase isoform
• pyrroline-5-carboxylate reductase 2
• pyrroline-5-carboxylate reductase family, member 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: PYCR2