Drebrin 1 Antibody (JE30-20)

Western Blot: Drebrin 1 Antibody (JE30-20) [NBP3-32279] - Western blot analysis of Drebrin 1 on different lysates with Rabbit anti-Drebrin 1 antibody (NBP3-32279) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: A431 cell lysate (low expression) Lane 4: Mouse brain tissue lysate Lane 5: AD Mouse brain tissue lysate (low expression) Lane 6: Rat brain tissue lysate Lane 7: PC-12 cell lysate Lane 8: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 71 kDa Observed band size: 110 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32279) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Drebrin 1 Antibody (JE30-20) [NBP3-32279] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Drebrin 1 antibody (NBP3-32279) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32279) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Drebrin 1 Antibody (JE30-20) [NBP3-32279] - Immunocytochemistry analysis of SH-SY5Y cells labeling Drebrin 1 with Rabbit anti-Drebrin 1 1 antibody (NBP3-32279) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Drebrin 1 antibody (NBP3-32279) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: Drebrin 1 Antibody (JE30-20) [NBP3-32279] - Flow cytometric analysis of SH-SY5Y cells labeling Drebrin 1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32279, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: JE30-20
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Drebrin 1 Antibody (JE30-20) Summary

• Description: Novus Biologicals Rabbit Drebrin 1 Antibody (JE30-20) (NBP3-32279) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human Drebrin 1 aa 100-200. (Uniprot: Q16643)
• Localization: Cytoplasm, Cell projection, dendrite, cell cortex, Cell junction, Cell projection, growth cone.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DBN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:250
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Drebrin 1 Antibody (JE30-20)

• D0S117E
• DBN1
• Developmentally-regulated brain protein
• DKFZp434D064
• Drba
• Drebrin 1
• Drebrin A
• Drebrin E
• Drebrin E2
• drebrin
• drebrin-1

Background

Drebrins are actin-binding proteins, that are developmentally regulated in the process of neuronal growth. Three isoforms, E1 and E2 (embryonic) and A (adult) are generated by alternative RNA splicing from a single debrin gene in the chicken brain. The expression pattern of each isoform is regulated spatially and temporally in the developing brain. It has been suggested that debrin is one of the intracellular regulators of the neuronal morphogenesis (4). Debrin has also been detected in other kinds of non-neuronal human and bovine tissue but its exact role is unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DBN1