Puromycin-sensitive aminopeptidase/NPEPPS Antibody

Western Blot: NPEPPS Antibody [NB100-93421] - (0.3ug/ml) Analysis of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: NPEPPS Antibody [NB100-93421] - Analysis of NPEPPS in Human Brain (Cerebral Cortex) lysate (35ug protein in RIPA buffer) at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Puromycin-sensitive aminopeptidase/NPEPPS Antibody Summary

• Description: Novus Biologicals Goat Puromycin-sensitive aminopeptidase/NPEPPS Antibody (NB100-93421) is a polyclonal antibody validated for use in WB and ELISA. Anti-Puromycin-sensitive aminopeptidase/NPEPPS Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KRDAESIHQYLLQRK corresponding to internal region according to NP_006301.2.
• Epitope: C-KRDAESIHQYLLQRK
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NPEPPS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.2 - 0.5 ug/ml
• Application Notes: WB: Approx. 110 kDa band observed in human brain (cerebral cortex) lysates (calculated MW of 98.5 kDa band according to NP_006301.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Puromycin-sensitive aminopeptidase/NPEPPS Antibody

• AAP-S
• aminopeptidase puromycin sensitive
• Cytosol alanyl aminopeptidase
• EC 3.4.11.2
• EC 3.4.11.3
• metalloproteinase MP100
• MP100
• MP100EC 3.4.11
• NPEPPS
• PSAEC 3.4.11.14
• puromycin-sensitive aminopeptidase

Background

This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Puromycin-sensitive aminopeptidase/NPEPPS Antibody (NB100-93421)(1)

Publication using NB100-93421

• Karsten SL, Sang TK, Gehman LT et al. A genomic screen for modifiers of tauopathy identifies puromycin-sensitive aminopeptidase as an inhibitor of tau-induced neurodegeneration. Neuron 2006-09-07 [PMID: 16950154]

Bioinformatics

• Gene Symbol: NPEPPS
• Entrez:
  • 9520(Human)
  • 19155(Mouse)
  • 50558(Rat)
• Uniprot:
  • NP_006301.2(Human)