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PUF60 Antibody (917)

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Western Blot: PUF60 Antibody (917) [NBP2-43764] - Analysis of A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [917] dilution: 1:1000.
Western Blot: PUF60 Antibody (917) [NBP2-43764] - Analysis of A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [917] dilution: 1:1000.
Immunohistochemistry-Paraffin: PUF60 Antibody (917) [NBP2-43764] - PUF60 antibody (917) detects PUF60 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PUF60 stained by PUF60 ...read more
Western Blot: PUF60 Antibody (917) [NBP2-43764] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell ...read more
Western Blot: PUF60 Antibody (917) [NBP2-43764] - Analysis of A. 50 ug mouse brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [917] dilution: 1:1000
Immunohistochemistry-Paraffin: PUF60 Antibody (917) [NBP2-43764] - PUF60 antibody (917) detects PUF60 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. PUF60 stained by PUF60 ...read more
Immunohistochemistry-Paraffin: PUF60 Antibody (917) [NBP2-43764] - PUF60 antibody (917) detects PUF60 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. PUF60 stained by PUF60 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC-P
Clone
917
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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PUF60 Antibody (917) Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PUF60. The exact sequence is proprietary.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PUF60
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for PUF60 Antibody (917)

  • FBP interacting repressor
  • FBP-interacting repressor
  • FIRpoly-U binding splicing factor PUF60
  • FLJ31379
  • FUSE-binding protein-interacting repressor
  • poly(U)-binding-splicing factor PUF60
  • poly-U binding splicing factor 60KDa
  • Ro ribonucleoprotein binding protein 1
  • Ro ribonucleoprotein-binding protein 1
  • Ro-binding protein 1
  • roBP1
  • RoBPI
  • siah binding protein 1,60 kDa poly(U)-binding-splicing factor
  • Siah-binding protein 1
  • Siah-BP1
  • SIAHBP1pyrimidine tract binding splicing factor

Background

PUF60 is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. PUF60 also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. PUF60 gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for PUF60 gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-302
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1003277) licensed under a CC-BY licence.
(4 Reviews)
(38 Publications)
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
XPB Antibody
NB100-61060
Immunoprecipitation: XPB Antibody [NB100-61060] - Detection of human ERCC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERCC3 antibody NB100-61060 used for IP at 3 ug per reaction. ERCC3 was also immunoprecipitated by rabbit anti-ERCC3 antibody NB100-61059. For blotting immunoprecipitated ERCC3, NB100-61060 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: XPB Antibody [NB100-61060] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiERCC3 antibody used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
(1 Review)
(3 Publications)
Species: Hu, Mu, V-Vi
Applications: ChIP, IP, WB
FUBP1 Antibody
NBP1-83918
Immunocytochemistry/Immunofluorescence: FUBP1 Antibody [NBP1-83918] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
GTF2H5
Overexpression Lysate
NBL1-11392
Western Blot: GTF2H5 Overexpression Lysate (Adult Normal) [NBL1-11392] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GTF2H5.
Species: Hu
Applications: WB
GTF2H4 Antibody
H00002968-B01P
Western Blot: GTF2H4 Antibody [H00002968-B01P] - Analysis of GTF2H4 expression in transfected 293T cell line by GTF2H4 polyclonal antibody. Lane 1: GTF2H4 transfected lysate(50.82 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: GTF2H4 Antibody [H00002968-B01P] - Analysis of purified antibody to GTF2H4 on HeLa cell. (antibody concentration 10 ug/ml)
Species: Hu
Applications: ICC/IF, WB
GTF2H2 Antibody
(PCRP-GTF2H2-1B9)
NBP3-07348
Protein Array: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348] - Analysis of Protein Array containing more than 19,000 full-length human proteins using GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9).
Flow Cytometry: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348] - Flow Cytometric Analysis of PFA-fixed HeLa cells. GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).
Immunocytochemistry/Immunofluorescence: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348] - Immunofluorescence Analysis of PFA-fixed HeLa cells. GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9) followed by goat anti- Mouse IgG-CF488 (green). Phalloidin counterstain (red).
Species: Hu
Applications: Flow, ICC/IF, PA
GTF2H3 Antibody
NBP2-87539
Immunohistochemistry-Paraffin: GTF2H3 Antibody [NBP2-87539] - Rabbit Anti-GTF2H3 antibody. Paraffin Embedded Tissue: Human Heart. Cellular Data: cardiac cell. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X
Western Blot: GTF2H3 Antibody [NBP2-87539] - WB Suggested Anti-GTF2H3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:2500. Positive Control: Jurkat cell lysateGTF2H3 is supported by BioGPS gene expression data to be expressed in Jurkat
Immunohistochemistry: GTF2H3 Antibody [NBP2-87539] - HumanMuscle
Species: Hu
Applications: IHC, IHC-P, WB
GTF2H1 Antibody
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H00002965-M01
Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.Lane 1: GTF2H1 transfected lysate(62 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.Antibody concentration 5 ug/ml.
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FBPase 1 Antibody
NBP1-84721
Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
(1 Publication)
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Applications: IHC, IHC-P, Simple Western, WB
RAB14 Antibody
NBP1-84720
Immunocytochemistry/Immunofluorescence: RAB14 Antibody [NBP1-84720] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Western Blot: RAB14 Antibody [NBP1-84720] - Analysis in human cell line TD47D.
Immunohistochemistry-Paraffin: RAB14 Antibody [NBP1-84720] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
(1 Publication)
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(548908) [Unconjugate
MAB5646
MCF‑7 human breast cancer cell line was stained with Mouse Anti‑Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Western blot shows lysates of human cortex tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FOLR1 at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. Use under non-reducing conditions only.
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AF5697
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Western blot shows lysates of human placental tissue. PVDF membrane was probed with 1 µg/mL of Human FOLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5697) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FOLR2 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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MARCH8 Antibody
NBP1-85540
Western Blot: MARCH8 Antibody [NBP1-85540] - Analysis in human cell line K562.
Immunohistochemistry-Paraffin: MARCH8 Antibody [NBP1-85540] - Staining of human Cerebellum shows strong granular cytoplasmic positivity in cells in molecular layer.
Immunohistochemistry-Paraffin: MARCH8 Antibody [NBP1-85540] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.
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MLXIP Antibody
NBP2-85297
Immunohistochemistry-Paraffin: MLXIP Antibody [NBP2-85297] - Rabbit Anti-MLXIP Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Adult heart. Observed Staining: Cytoplasmic. Primary Antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy2/3. Secondary Antibody Concentration: 1:200. Magnification: 20X.
Western Blot: MLXIP Antibody [NBP2-85297] - WB Suggested Anti-MLXIP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: PANC1 cell lysateMLXIP is supported by BioGPS gene expression data to be expressed in PANC1
Western Blot: MLXIP Antibody [NBP2-85297] - Host: Rabbit. Target Name: MLXIP. Sample Type: Human Fetal Heart. Antibody Dilution: 1.0ug/ml
Species: Hu
Applications: IHC, IHC-P, WB
MYLIP Antibody
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NBP2-46069
Western Blot: MYLIP Antibody (OTI3A2) [NBP2-46069] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYLIP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYLIP.
(1 Publication)
Species: Hu, Mu, Rt
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SAP155 Antibody
NBP2-47291
Immunocytochemistry/Immunofluorescence: SAP155 Antibody [NBP2-47291] - Immunofluorescent staining of human cell line HeLa shows localization to nuclear speckles.
Western Blot: SAP155 Antibody [NBP2-47291] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunohistochemistry-Paraffin: SAP155 Antibody [NBP2-47291] - Immunohistochemical staining of human salivary gland shows strong nuclear and cytoplasmic positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
HO-1/HMOX1/HSP32
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Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Analysis of 10^6 Jurkat cells stained using HO-1 monoclonal antibody (HO-1-1), (Prod. No. ADI-OSA-110), at a concentration of 50 ug/mL
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Analysis of human spleen tissue stained with HO-1, mAb (HO-1-1) at 10ug/ml.
Western Blot: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Lane 1: MW marker; Lane 2: HO-1 (rat), (recombinant); Lane 3: HO-1 (human), (recombinant); Lane 4: HO-2 (human), (recombinant); Lane 5: MDBK cell lysate; Lane 6: Dog liver microsome; Lane 7: Mouse liver microsome.
(1 Review)
(18 Publications)
Species: Bv, Ca, Hu, Mu, Rt
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FARP2 Antibody
NBP1-84680
Western Blot: FARP2 Antibody [NBP1-84680] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: FARP2 Antibody [NBP1-84680] - Staining of human liver shows distinct cytoplasmic positivity in hepatocytes.
Species: Hu
Applications: IHC, IHC-P, WB
Calreticulin
Antibody - BSA Free
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Knockdown Validated: Calreticulin Antibody [NB600-101] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.
Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-101] - The Calreticulin antibody NB600-101 was tested in HeLa cells at a 1:250 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue), respectively.
(27 Publications)
Species: Bv, Ha, Hu, Mu, Pm, Rt
Applications: B/N, DB, EM, Flow, ICC/IF, IHC, IHC-P, IP, KD, PA, Simple Western, WB
SSB Antibody
(OTI2C8)
NBP1-48002
Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SSB mouse monoclonal antibody.
Species: Ca, Hu, Pm, Pm
Applications: ICC/IF, IHC, IHC-P, IP, WB

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Bioinformatics

Gene Symbol PUF60