PUF60 Antibody (677) - Azide and BSA Free

Western Blot: PUF60 Antibody (677) [NBP2-43836] - Analysis of A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [677] dilution: 1:1000

Immunocytochemistry/Immunofluorescence: PUF60 Antibody (677) [NBP2-43836] - Analysis of A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PUF60 protein stained by PUF60 antibody [677] diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: PUF60 Antibody (677) [NBP2-43836] - AGS xenograft. PUF60 antibody [677] dilution: 1:100. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: PUF60 Antibody (677) [NBP2-43836] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE PUF60 antibody [677] dilution: 1:5000

Western Blot: PUF60 Antibody (677) [NBP2-43836] - Analysis of A. 50 ug mouse brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [677] dilution: 1:1000

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 677
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PUF60 Antibody (677) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PUF60 Antibody (677) - Azide and BSA Free (NBP2-43836) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen used to generate this antibody corresponds to human PUF60
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PUF60
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for PUF60 Antibody (677) - Azide and BSA Free

• FBP interacting repressor
• FBP-interacting repressor
• FIRpoly-U binding splicing factor PUF60
• FLJ31379
• FUSE-binding protein-interacting repressor
• poly(U)-binding-splicing factor PUF60
• poly-U binding splicing factor 60KDa
• Ro ribonucleoprotein binding protein 1
• Ro ribonucleoprotein-binding protein 1
• Ro-binding protein 1
• roBP1
• RoBPI
• siah binding protein 1,60 kDa poly(U)-binding-splicing factor
• Siah-binding protein 1
• siah-BP1
• SIAHBP1pyrimidine tract binding splicing factor

Background

PUF60 is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. PUF60 also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. PUF60 gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for PUF60 gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PUF60