PUF60 Antibody (1C1) - Azide and BSA Free

Western Blot: PUF60 Antibody (1C1) [H00022827-M20] - Analysis of PUF60 expression in transfected 293T cell line by PUF60 monoclonal antibody (M20), clone 1C1. Lane 1: PUF60 transfected lysate (Predicted MW: 59.9 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: PUF60 Antibody (1C1) [H00022827-M20] - Analysis of monoclonal antibody to PUF60 on HeLa cell. Antibody concentration 10 ug/ml

Sandwich ELISA: PUF60 Antibody (1C1) [H00022827-M20] - Detection limit for recombinant GST tagged PUF60 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PUF60 Antibody (1C1) - Azide and BSA Free Summary

• Immunogen: PUF60 (AAH11979, 1 a.a. ~ 516 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA
• Specificity: Reacts with poly-U binding splicing factor 60KDa.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PUF60
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: This antibody is reactive against recombinant protein in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PUF60 Antibody (1C1) - Azide and BSA Free

• FBP interacting repressor
• FBP-interacting repressor
• FIRpoly-U binding splicing factor PUF60
• FLJ31379
• FUSE-binding protein-interacting repressor
• poly(U)-binding-splicing factor PUF60
• poly-U binding splicing factor 60KDa
• Ro ribonucleoprotein binding protein 1
• Ro ribonucleoprotein-binding protein 1
• Ro-binding protein 1
• roBP1
• RoBPI
• siah binding protein 1,60 kDa poly(U)-binding-splicing factor
• Siah-binding protein 1
• siah-BP1
• SIAHBP1pyrimidine tract binding splicing factor

Background

The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PUF60
• Entrez:
  • 22827(Human)
• Uniprot:
  • AAH11979(Human)