PTPRB Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: PTPRB Antibody [NBP2-88829] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: PTPRB Antibody [NBP2-88829] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

PTPRB Antibody - BSA Free Summary

• Immunogen: Peptide with sequence C-ENHSFSQERTVPDK corresponding to internal region according to NP_001103224.1, NP_002828.3.
• Epitope: ENHSFSQERTVPDK - internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTPRB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:16000

Reactivity Notes

Expected species reactivity based on sequence similarity: Rat, Porcine, Canine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.5 mg/ml in Tris saline pH7.3, 0.5% bovine serum albumin
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PTPRB Antibody - BSA Free

• DKFZp686E2262
• DKFZp686H15164
• EC 3.1.3.48
• FLJ44133
• HPTPB
• HPTP-BETA
• MGC142023
• MGC59935
• protein tyrosine phosphatase, receptor type, B
• protein tyrosine phosphatase, receptor type, beta polypeptide
• Protein-tyrosine phosphatase beta
• PTPB
• receptor-type tyrosine-protein phosphatase beta
• R-PTP-BETA
• Vascular endothelial protein tyrosine phosphatase
• VEPTP
• VE-PTP

Background

PTPRB is a member of the protein tyrosine phosphatase (PTP) family. The family comprises at least 37 proteins, characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. PTP1B is a transmembrane PTP. The PTPs have high substrate specificity for phosphotyrosyl proteins, at the primary sequence level sharing little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP beta interacts with and regulates the tyrosine phosphorylation level of catenins, which are critical in physiological and pathological events such as cell migration, adhesion and transformation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRB