PTPN13/PTPL1 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PTPN13/PTPL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3577) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTPN13/PTPL1 at approximately 260 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PTPN13 regulates MDA-MB-231 cell motility and invasiveness. A: Expression of wt (N13-1, N13-2, N13-3) or catalytically inactive (CS) PTPN13 in the indicated cell clones was monitored by western blotting using anti-PTPN13 antibodies. Mock: control cells (vector alone); equal loading was verified by re-probing with an anti-actin antibody. B: Cell growth measured using the MTS assay. Results, expressed as % of Mock cells, are the mean ± s.d. of three (N13-1) four (N13-2, N13-3) or five (Mock, CS) independent experiments. C: Directional migration was assessed with the wound healing assay. C. Upper panel: Phase-contrast optical photomicrographs of the wounded area at 0 and 9h. C. Lower panel: Quantification of cell migration, expressed as % of Mock cells; mean ± s.d. of 3 (N13-1) or ≥5 (Mock, N13-2, N13-3, CS) independent experiments. **P40µm/h). F: Cell invasiveness was evaluated with the Boyden chamber test. The percentage of cells that migrated through Matrigel-coated filters was quantified relative to the total number of seeded cells. Results, expressed as % of Mock cells, are the mean ± s.d. of five (N13-1), three (N13-2, N13-3) or two (CS) independent experiments *P<0.05 versus Mock. (C, D and F) two-tailed Student's t-test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31938048), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PTPN13/PTPL1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PTPN13/PTPL1
  Met1-Arg500
  Accession # Q12923
• Specificity: Detects endogenous human PTPN13/PTPL1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTPN13
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTPN13/PTPL1 Antibody [Unconjugated]

• EAP-1
• FAP-1
• hPTPE1
• PNP1
• protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associatedphosphatase)
• protein-tyrosine phosphatase 1, Fas-associated
• PTP1E
• PTP1EDKFZp686J1497
• PTP-BAS
• PTP-BASEC 3.1.3.48
• PTP-BL
• PTP-E1
• PTPL1
• PTPL1nonreceptor type 13
• PTPLE
• PTPN13
• tyrosine-protein phosphatase non-receptor type 13

Background

Protein Tyrosine Phosphatase, Non-receptor Type 13, also called PTPN13, PTPL1, FAP-1, hPTP1E and PTP-BAS, is a 260 kDa intracellular protein that removes phosphates from tyrosine residues. Genetic aberrations in PTPN13 have been reported in a wide variety of cancers, including, bone, colon, hepatocellular, and germ cell line. Elevated levels of PTPN13 in cancer cell lines have also been correlated with resistance to Fas-induced apoptosis, possibly due to prevention of CD95 reaching the cell surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTPN13/PTPL1 Antibody (AF3577)(2)

Publications using AF3577

• Mohamed Hamyeh, Florence Bernex, Romain M. Larive, Aurélien Naldi, Serge Urbach, Joelle Simony-Lafontaine et al. PTPN13 induces cell junction stabilization and inhibits mammary tumor invasiveness Theranostics 2020-01-01 [PMID: 31938048]
• Ajay Mishra, Bénédicte Oulès, Angela Oliveira Pisco, Tony Ly, Kifayathullah Liakath-Ali, Gernot Walko et al. A protein phosphatase network controls the temporal and spatial dynamics of differentiation commitment in human epidermis eLife 2017-10-18 [PMID: 29043977]

Bioinformatics

• Gene Symbol: PTPN13
• Uniprot:
  • Q12923()