PTP1B/PTPN1 Antibody (OTI1D10)

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and PTPN1-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PTPN1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control (1:500). Image using the Azide and BSA Free format of this antibody.

Immunocytochemistry/Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN1.

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - Staining of paraffin-embedded Human lymphoma tissue using anti-PTPN1 mouse monoclonal antibody.

Flow Cytometry: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PTPN1 antibody, and then analyzed by flow cytometry.

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1.

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - Analysis of extracts (35ug) from 9 different cell lines by using anti-PTPN1 monoclonal antibody.

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - Staining of paraffin-embedded Human colon tissue using anti-PTPN1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - Staining of paraffin-embedded Human lymph node tissue using anti-PTPN1 mouse monoclonal antibody.

• Reactivity: Hu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, KO
• Clone: OTI1D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PTP1B/PTPN1 Antibody (OTI1D10) Summary

• Immunogen: Full length human recombinant protein of human PTPN1(NP_002818) produced in HEK293 cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Knockout Validated
  • Western Blot 1:500-2000
• Theoretical MW: 49.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PTP1B/PTPN1 Antibody (OTI1D10)

• EC 3.1.3.48
• protein tyrosine phosphatase 1B
• protein tyrosine phosphatase, non-receptor type 1
• Protein-tyrosine phosphatase 1B
• PTP1B
• PTP-1B
• PTP1Bprotein tyrosine phosphatase, placental
• PTPN1
• tyrosine-protein phosphatase non-receptor type 1

Background

The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPN1
• Entrez:
  • 5770(Human)