PTP1B/PTPN1 Antibody (208607) [Unconjugated]

• Clone: 208607
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PTP1B/PTPN1 Antibody (208607) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PTP1B
  Glu2-Thr435
  Accession # P18031
• Specificity: Detects human PTP1B in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. The recombinant protein lacks the C-terminal 114 amino acids but is fully active. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta -catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness.

1. Angers-Loustau, et al. (1999) Biochem. Cell Biol. 77:493.
2. Sarmiento, et al. (1998) J. Biol. Chem. 273:26368.
3. Bjorge, et al. (2000) J. Biol. Chem. 52:41439.
4. Balsamo, et al. (1996) J. Cell Biol. 134:801.
5. Elchebly, et al. (1999) Science 283:1544.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.