PTP beta/zeta/PTPRZ Antibody (279245) [Unconjugated]

PTP beta /zeta/PTPRZ was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Rat Anti-Human PTP beta /zeta/PTPRZ Monoclonal Antibody (Catalog # MAB26881) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 279245
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

PTP beta/zeta/PTPRZ Antibody (279245) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PTP beta / zeta /PTPRZ
  Gly302-Asn754
  Accession # P23471
• Specificity: Detects human PTP beta / zeta /PTPRZ in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PTPRZ1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTP beta/zeta/PTPRZ Antibody (279245) [Unconjugated]

• 3F8 CSPG
• HPTPZ
• phosphacan
• protein tyrosine phosphatase, receptor-type, Z polypeptide 1
• PTP beta/zeta
• PTP18
• PTPRZ
• PTPRZ1
• PTPZ
• receptor-type, zeta polypeptide 1
• RPTPB
• RPTPbeta

Background

PTPRZ, also known as Protein tyrosine phosphatase beta/zeta (PTP beta / zeta ), is a member of the receptor-type protein-tyrosine phosphatase family. This phosphatase is involved in the regulation of specific developmental processes in the central nervous system. A protein of 2,307 amino acids, PTPRZ is membrane-spanning with two cytoplasmic protein tyrosine phosphatase domains and an extracellular domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTP beta/zeta/PTPRZ Antibody (MAB26881)(2)

Publications using MAB26881

• José Javier Miguel-Hidalgo, Erik Hearn, Mohadetheh Moulana, Khunsa Saleem, Austin Clark, Maggie Holmes et al. Reduced length of nodes of Ranvier and altered proteoglycan immunoreactivity in prefrontal white matter in major depressive disorder and chronically stressed rats Sci Rep 9/29/2023 [PMID: 37775676]
• Y Shi, YF Ping, W Zhou, ZC He, C Chen, BS Bian, L Zhang, L Chen, X Lan, XC Zhang, K Zhou, Q Liu, H Long, TW Fu, XN Zhang, MF Cao, Z Huang, X Fang, X Wang, H Feng, XH Yao, SC Yu, YH Cui, X Zhang, JN Rich, S Bao, XW Bian Tumour-associated macrophages secrete pleiotrophin to promote PTPRZ1 signalling in glioblastoma stem cells for tumour growth Nat Commun, 2017-06-01;8(0):15080. 2017-06-01 [PMID: 28569747] (Flow Cytometry, Functional Assay, Human, Mouse)

Bioinformatics

• Gene Symbol: PTPRZ1
• Uniprot:
  • P23471()