Recombinant Human PTHLH/PTHrP Protein

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SDS-Page: Human PTHLH/PTHrP Protein [NBP2-35224]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Format
Carrier-Free
Concentration
LYOPH

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Catalog# & Formulation Size Price

Recombinant Human PTHLH/PTHrP Protein Summary

Description
A single, non-glycosylated biologically active polypeptide chain corresponding to 86 residues of PTHLH.

Source: E. coli

Amino Acid Sequence: AVSEHQLLHD KGKSIQDLRR RFFLHHLIAE IHTAEIRATS EVSPNSKPSP NTKNHPVRFG SDDEGRYLTQ ETNKVETYKE QPLKTP

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PTHLH
Purity
> 98 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
9.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 10 mM PB, pH 6.0, 300 mM NaCl.
Concentration
LYOPH
Purity
> 98 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human PTHLH/PTHrP Protein

  • BDE2
  • HHM
  • MGC14611
  • Osteostatin
  • parathyroid hormone-like hormone
  • Parathyroid hormone-like protein
  • parathyroid hormone-related protein
  • PLPosteostatin
  • PTHLH
  • PTHR
  • PTH-related protein
  • PTHRP
  • PTH-rP
  • PTHRPparathyroid hormone-like related protein

Background

PTHLH (PTHrP), also named parathyroid hormone-related protein, is belonging to the parathyroid hormone family. PTHLH (PTHrP) is expressed in cancer cells (breast cancer, certain types of lung cancer including squamous cell lung carcinoma). The receptor for PTHrP is PTHR1. PTHLH (PTHrP) plays a central role in regulating the hypercalcemia. Recombinant human PTHLH (PTHrP) is a 9.9 kDa linear polypeptide of 86 amino acid residues and it shares 86 % a.a. identity with murine PTHLH.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PTHLH
Uniprot