PTH Antibody (918462) [Alexa Fluor® 350]

• Applications: WB, IHC
• Clone: 918462
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

PTH Antibody (918462) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human PTH
  Ser32-Gln115
  Accession # P01270
• Specificity: Detects human PTH in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

PTH (Parathyroid Hormone) is a critical hormone in the regulation of Ca⁺⁺ homeostasis (1). The human PTH cDNA encodes 115 amino acids (aa) including a 25 aa signal sequence, a 6 aa propeptide, and an 84 aa mature hormone. Mature human PTH shares 70%, 73%, 88%, 87%, 86%, 86% and 85% aa identity with mouse, rat, canine, equine, bovine, porcine and feline PTH, respectively. Multiple N‑terminal peptides and C-terminal peptides derived from PTH occur naturally in the circulation (1). PTH aa 32-66, called PTH (1-34) since it represents the first 34 aa of the mature hormone, reproduces all the activity of the full length mature hormone and has been used therapeutically for treatment of osteoporosis (1-3). C-terminal peptides mainly oppose the activities of PTH (1-34) and are increased in renal failure (1-3). PTH expression is mainly restricted to the parathyroid gland, with minor amounts in the thymus (4). PTH secretion is enhanced by low Ca⁺⁺ concentrations and inhibited by FGF-23 (1, 5). In normal human plasma, PTH correlates negatively with active Vitamin D and positively with ionized calcium (6). Human and other mammalian PTH will bind and stimulate human or rat PTH1R, activating adenylate cyclase and increasing cAMP production (2, 7). PTH promotes secretion of TRANCE/RANKL and periostin through PTH1R binding on osteoblasts and/or bone marrow stromal cells (8-10). TRANCE/RANKL induces differentiation of osteoclasts, which in turn promote release of Ca⁺⁺ from bone (1, 8, 9). PTH1R on osteocytes, however, allows PTH to promote bone formation and IGF-1 production (11, 12). In renal epithelium, PTH promotes conversion of Vitamin D to its active form, lowers Ca⁺⁺ excretion and increases phosphate excretion (1, 2, 9). PTH also increases hematopoietic stem cell proliferation and mobilization and induces arterial vasodilation by regulating Ca⁺⁺ influx in PTH1R-expressing arterial smooth muscle (8, 13).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.