PLUNC Antibody

Images

 
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse PLUNC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4274) followed by HRP-conjugated Anti-Sheep ...read more
PLUNC was detected in immersion fixed frozen sections of mouse embryo nose (13 d.p.c.) using Sheep Anti-Mouse PLUNC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4274) at 1.7 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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PLUNC Antibody Summary

Immunogen
E. coli-derived recombinant mouse PLUNC
Ala59-Val278
Accession # NP_035256
Specificity
Detects mouse PLUNC in direct ELISAs and Western blots. In these formats, less than 10% cross-reactivity with recombinant human PLUNC is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
BPIFA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF4274 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLUNC Antibody

  • bA49G10.5
  • BPIFA1
  • ligand-binding protein RYA3
  • LPLUNC3
  • Lung-specific protein X
  • LUNX
  • LUNXNASG
  • Nasopharyngeal carcinoma-related protein
  • Palate lung and nasal epithelium clone protein
  • palate, lung and nasal epithelium associated
  • palate, lung and nasal epithelium carcinoma associated
  • PLUNC
  • protein Plunc
  • Secretory protein in upper respiratory tracts
  • SPLUNC1
  • SPLUNC1SPURT
  • tracheal epithelium enriched protein
  • Tracheal epithelium-enriched protein
  • Von Ebner protein Hl

Background

PLUNC (Palate, lung, and nasal epithelium clone; also SPLUNC1) is a 25-30 kDa member of the PLUNC family, BPI/PLUNC/LBP superfamily of proteins. PLUNC is a secreted glycoprotein that is expressed by both adult and embryonic respiratory epithelium in the nose, trachea and bronchi/primary bronchioles. It serves as a natural antibacterial polypeptide in the respiratory system. Mature mouse PLUNC is 259 amino acids (aa) in length. It contains four, six-amino acid repeats (GxxLPL) over aa 23-52. There is reportedly one splice variant that shows a 28 aa substitution for the C-terminal 45 amino acids (aa 234-278). Over aa 59-278, mouse PLUNC shares 91% and 75% aa identity with rat and human PLUNC, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PLUNC Antibody (AF4274)(3)

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF4274

Additional PLUNC Products

Bioinformatics Tool for PLUNC Antibody (AF4274)

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Bioinformatics

Gene Symbol BPIFA1
Uniprot