PSME4 Antibody - BSA Free

Western Blot: PSME4 Antibody - BSA Free [NBP3-46861] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (PSME4 antibody) at dilution of 1:400

Immunoprecipitation: PSME4 Antibody - BSA Free [NBP3-46861] - IP Result of anti-PSME4 (IP:, 3ug; Detection: 1:500) with HEK-293 cells lysate 1760ug.

Immunohistochemistry: PSME4 Antibody - BSA Free [NBP3-46861] - Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using ( PSME4 antibody) at dilution of 1:200

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PSME4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PSME4 Antibody - BSA Free (NBP3-46861) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: proteasome(prosome, macropain) activator subunit 4. (Uniprot# Q14997)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSME4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:100
  • Immunohistochemistry 1:50-1:500
  • Immunoprecipitation 1:500-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 140 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PSME4 Antibody - BSA Free

• proteasome (prosome, macropain) activator subunit 4

Background

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator. PA200 has been identified as a 200 kDa nuclear protein that activates the 26S proteasome complex. Studies have shown that, in human tissues, PA200 mRNA levels are highest in testis, but is also present in liver, spleen, brain, heart, kidney and lung. Evidence suggests that this protein is involved in DNA repair, possibly by recruiting the proteasome to double-strand DNA breaks.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSME4