PSME4 Antibodies
We offer PSME4 Antibodies for use in common research applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot. Each PSME4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PSME4 Antibodies can be used in a variety of model species: Bovine, Human, Mouse, Rat. Use the list below to choose the PSME4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PSME4 polyclonal antibodies.
Alternate Names for PSME4 Antibodies
anti-PSME4 antibody, anti-proteasome (prosome, macropain) activator subunit 4 antibody
5 Results for "psme4" in Primary Antibodies
![Western Blot: PSME4 Antibody [NBP2-22236] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22236 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PSME4-Antibody-Western-Blot-NBP2-22236-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PSME4 Antibody [NBP2-22236] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22236 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: PSME4 Antibody [NBP2-22236] - Human non-small cell lung cancer. Antibody: Affinity purified rabbit anti- PSME4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/PSME4-Antibody-Immunohistochemistry-Paraffin-NBP2-22236-img0007.jpg)
![Immunoprecipitation: PSME4 Antibody [NBP2-22236] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. NBP2-22236 used for IP at 6 ug/mg lysate. PSME4 was also immunoprecipitated by rabbit anti-PSME4 antibody. For blotting immunoprecipitated PSME4, NBP2-22236 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PSME4-Antibody-Immunoprecipitation-NBP2-22236-img0002.jpg)
![Western Blot: PSME4 Antibody [NBP2-94690] - Analysis of extracts of various cell lines, using PSME4 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PSME4-Antibody-Western-Blot-NBP2-94690-img0001.jpg "Western Blot: PSME4 Antibody [NBP2-94690] - Analysis of extracts of various cell lines, using PSME4 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: PSME4 Antibody [NBP2-94690] - Immunohistochemistry of paraffin-embedded Human esophageal using PSME4 Rabbit pAb (NBP2-94690) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PSME4-Antibody-Immunohistochemistry-Paraffin-NBP2-94690-img0005.jpg)
![Immunohistochemistry-Paraffin: PSME4 Antibody [NBP2-94690] - Mouse heart using PSME4 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PSME4-Antibody-Immunohistochemistry-Paraffin-NBP2-94690-img0003.jpg)
![Immunohistochemistry-Paraffin: PSME4 Antibody [NBP2-94690] - Immunohistochemistry of paraffin-embedded Rat kidney using PSME4 Rabbit pAb (NBP2-94690) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PSME4-Antibody-Immunohistochemistry-Paraffin-NBP2-94690-img0004.jpg)
![Western Blot: PSME4 Antibody - BSA Free [NBP3-46861] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (PSME4 antibody) at dilution of 1:400](http://images.novusbio.com/fullsize/nbp3-46861_rabbit-psme4-pab-bsa-free-284202512315782.jpg "Western Blot: PSME4 Antibody - BSA Free [NBP3-46861] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (PSME4 antibody) at dilution of 1:400")
![Immunoprecipitation: PSME4 Antibody - BSA Free [NBP3-46861] - IP Result of anti-PSME4 (IP:, 3ug; Detection: 1:500) with HEK-293 cells lysate 1760ug.](http://images.novusbio.com/fullsize/nbp3-46861_rabbit-psme4-pab-bsa-free-28420251235957.jpg)
![Immunohistochemistry: PSME4 Antibody - BSA Free [NBP3-46861] - Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using ( PSME4 antibody) at dilution of 1:200](http://images.novusbio.com/fullsize/nbp3-46861_rabbit-psme4-pab-bsa-free-284202512283357.jpg)
![Immunocytochemistry/Immunofluorescence: PSME4 Antibody [NBP2-32575] - Staining of human cell line Hep G2 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PSME4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32575-img0009.jpg "Immunocytochemistry/Immunofluorescence: PSME4 Antibody [NBP2-32575] - Staining of human cell line Hep G2 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Western Blot: PSME4 Antibody [NBP2-32575] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/antibody/nbp2-32575_rabbit-polyclonal-psme4-antibody-western-blot-1610202491919..jpg)
![Western Blot: PSME4 Antibody [NBP3-32869] - Western blot analysis of PSME4 on different lysates with Rabbit anti-PSME4 antibody (NBP3-32869) at 1/500 dilution. Lane 1: 293T cell lysate (10 ug/Lane) Lane 2: Hela cell lysate (10 ug/Lane) Lane 3: Jurkat cell lysate (10 ug/Lane) Lane 4: RAW264.7 cell lysate (10 ug/Lane) Lane 5: Rat skeletal muscle tissue lysate (20 ug/Lane) Predicted band size: 211 kDa Observed band size: 211 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32869) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32869_rabbit-psme4-pab-288202412442999.jpg "Western Blot: PSME4 Antibody [NBP3-32869] - Western blot analysis of PSME4 on different lysates with Rabbit anti-PSME4 antibody (NBP3-32869) at 1/500 dilution. Lane 1: 293T cell lysate (10 ug/Lane) Lane 2: Hela cell lysate (10 ug/Lane) Lane 3: Jurkat cell lysate (10 ug/Lane) Lane 4: RAW264.7 cell lysate (10 ug/Lane) Lane 5: Rat skeletal muscle tissue lysate (20 ug/Lane) Predicted band size: 211 kDa Observed band size: 211 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32869) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PSME4 Antibody [NBP3-32869] - Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-PSME4 antibody (NBP3-32869) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32869) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32869_rabbit-psme4-pab-298202414262659.jpg)