PSMD12 Antibody

Western Blot: PSMD12 Antibody [NBP2-19986] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 C: U87-MG 10% SDS PAGE gel, diluted at 1:10000.

Immunohistochemistry-Paraffin: PSMD12 Antibody [NBP2-19986] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.

Product Discontinued

PSMD12 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PSMD12. The exact sequence is proprietary.
• Predicted Species: Mouse (99%), Rat (99%), Chicken (93%), Xenopus (92%), Rhesus Macaque (100%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSMD12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (92%), Zebrafish (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for PSMD12 Antibody

• 26S proteasome non-ATPase regulatory subunit 12
• 26S proteasome regulatory subunit p55
• 26S proteasome regulatory subunit RPN5
• MGC75406
• p55
• proteasome (prosome, macropain) 26S subunit, non-ATPase, 12
• Rpn5

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PSMD12
• Uniprot:
  • O00232(Human)