PSMB10/MECL1 Antibody

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Western Blot: PSMB10/MECL1 Antibody [NBP1-51958] - Analysis of PSMB10 in Human Lung lysate (35ug protein in RIPA buffer) using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PSMB10/MECL1 Antibody Summary

Immunogen
Peptide with sequence C-PTEPVKRSGRYH corresponding to internal region according to NP_002792.1.
Epitope
C-PTEPVKRSGRYH
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PSMB10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 26 kDa band observed in human, liver, lung and lymph node lysates (calculated MW of 28.9 kDa band according to NP_002792.1). IHC-P: Human tonsil shows efined staining in a subset of cells.
Publications
Read Publication using NBP1-51958.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PSMB10/MECL1 Antibody

  • beta2i
  • FLJ00366
  • LMP10
  • LMP10Proteasome subunit beta-2i
  • Low molecular mass protein 10
  • Macropain subunit MECl-1
  • MECL1
  • MECl-1
  • MECL1EC 3.4.25.1
  • MGC1665
  • Multicatalytic endopeptidase complex subunit MECl-1
  • proteasome (prosome, macropain) subunit, beta type, 10
  • proteasome catalytic subunit 2i
  • Proteasome MECl-1
  • proteasome subunit beta 7i
  • proteasome subunit beta type-10
  • Proteasome subunit beta-2i
  • proteasome subunit MECL1
  • PSMB10

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSMB10/MECL1 Antibody (NBP1-51958)(1)

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Bioinformatics

Gene Symbol PSMB10
Entrez
Uniprot