PSMB10/MECL1 Antibody

Western Blot: PSMB10/MECL1 Antibody [NBP1-51958] - Analysis of PSMB10 in Human Lung lysate (35 ug protein in RIPA buffer) using this antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-51958] - Staining of paraffin embedded human small intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-51958] - Staining of paraffin embedded human tonsil. Antbody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PSMB10/MECL1 Antibody Summary

• Immunogen: Peptide with sequence C-PTEPVKRSGRYH corresponding to internal region according to NP_002792.1.
• Epitope: C-PTEPVKRSGRYH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PSMB10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: Western blot: Approx 26 kDa band observed in Human, Liver, Lung and Lymph Node lysates (calculated MW of 28.9 kDa according to NP_002792.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PSMB10/MECL1 Antibody

• beta2i
• FLJ00366
• LMP10
• LMP10Proteasome subunit beta-2i
• Low molecular mass protein 10
• Macropain subunit MECl-1
• MECL1
• MECl-1
• MECL1EC 3.4.25.1
• MGC1665
• Multicatalytic endopeptidase complex subunit MECl-1
• proteasome (prosome, macropain) subunit, beta type, 10
• proteasome catalytic subunit 2i
• Proteasome MECl-1
• proteasome subunit beta 7i
• proteasome subunit beta type-10
• Proteasome subunit beta-2i
• proteasome subunit MECL1
• PSMB10

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSMB10/MECL1 Antibody (NBP1-51958)(1)

Publication using NBP1-51958

• Moschonas A, Kouraki M, Knox PG et al. CD40 induces antigen transporter and immunoproteasome gene expression in carcinomas via the coordinated action of NF-kappaB and of NF-kappaB-mediated de novo synthesis of IRF-1. Mol Cell Biol 2008-05-01 [PMID: 18694960]

Bioinformatics

• Gene Symbol: PSMB10
• Entrez:
  • 5699(Human)
  • 19171(Mouse)
  • 291983(Rat)
• Uniprot:
  • NP_002792.1(Human)