PSMA/FOLH1/NAALADase I Antibody (OTI3H5) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) - Azide and BSA Free [NBP2-73686] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FOLH1.

Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) - Azide and BSA Free [NBP2-73686] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FOLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Flow Cytometry: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) - Azide and BSA Free [NBP2-73686] - HEK293T cells transfected with either RC218310 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FOLH1 antibody and then analyzed by flow cytometry.

Western Blot: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) - Azide and BSA Free [NBP2-73686] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-FOLH1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Western Blot: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) - Azide and BSA Free [NBP2-73686] - Transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA.

Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) - Azide and BSA Free [NBP2-73686] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FOLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI3H5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PSMA/FOLH1/NAALADase I Antibody (OTI3H5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PSMA/FOLH1/NAALADase I Antibody (OTI3H5) - Azide and BSA Free (NBP2-02045) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human FOLH1(NP_004467) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOLH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-1000
• Theoretical MW: 84.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PSMA/FOLH1/NAALADase I Antibody (OTI3H5) - Azide and BSA Free

• Cell growth-inhibiting gene 27 protein
• cell growth-inhibiting protein 27
• EC 3.4.17.21
• FGCP
• folate hydrolase (prostate-specific membrane antigen) 1
• Folate hydrolase 1
• FOLH1
• FOLHNAALADase I
• Folylpoly-gamma-glutamate carboxypeptidase
• GCP2
• GCPII
• GCPIImGCP
• glutamate carboxylase II
• glutamate carboxypeptidase 2
• Glutamate carboxypeptidase II
• Membrane glutamate carboxypeptidase
• mopsm
• NAALAD1
• NAALAD1N-acetylated-alpha-linked acidic dipeptidase I
• NAALADase I
• NAALAdase
• N-acetylated alpha-linked acidic dipeptidase 1
• prostate specific membrane antigen variant F
• Prostate-specific membrane antigen
• PSMA
• PSMAFGCP
• PSMPteroylpoly-gamma-glutamate carboxypeptidase

Background

PSMA, also known as Glutamate carboxypeptidase 2, is a 84kDa 750 amino acid protein that has 8 isoforms produced by alternative splicing. The primary function of this protein is to act as a glutamate carboxypeptidase. Current research on PSMA is being conducted in relation to prostate cancer, muscular atrophy, small cell carcinoma, coronary heart disease and Huntington's disease. PSMA is known to interact with MME, MTAP and RPS3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOLH1