PSMA/FOLH1/NAALADase I Antibody (OTI3H5)

Western Blot: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PSMA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMA.

Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PSMA mouse monoclonal antibody.

Flow Cytometry: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PSMA antibody, and then analyzed by flow cytometry.

Western Blot: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045] - Transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA.

Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA mouse monoclonal antibody.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, Flow, ICC/IF, IHC
• Clone: OTI3H5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.71 mg/ml

PSMA/FOLH1/NAALADase I Antibody (OTI3H5) Summary

• Description: Novus Biologicals Mouse PSMA/FOLH1/NAALADase I Antibody (OTI3H5) (NBP2-02045) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and Simple Western. Anti-PSMA/FOLH1/NAALADase I Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human FOLH1(NP_004467) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOLH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Simple Western 1:50
  • Western Blot 1:500-1000
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Tested in Prostate cell lysates, antibody dilution of 1:50
• Theoretical MW: 84.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.71 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PSMA/FOLH1/NAALADase I Antibody (OTI3H5)

• Cell growth-inhibiting gene 27 protein
• cell growth-inhibiting protein 27
• EC 3.4.17.21
• FGCP
• folate hydrolase (prostate-specific membrane antigen) 1
• Folate hydrolase 1
• FOLH1
• FOLHNAALADase I
• Folylpoly-gamma-glutamate carboxypeptidase
• GCP2
• GCPII
• GCPIImGCP
• glutamate carboxylase II
• glutamate carboxypeptidase 2
• Glutamate carboxypeptidase II
• Membrane glutamate carboxypeptidase
• mopsm
• NAALAD1
• NAALAD1N-acetylated-alpha-linked acidic dipeptidase I
• NAALADase I
• NAALAdase
• N-acetylated alpha-linked acidic dipeptidase 1
• prostate specific membrane antigen variant F
• Prostate-specific membrane antigen
• PSMA
• PSMAFGCP
• PSMPteroylpoly-gamma-glutamate carboxypeptidase

Background

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSMA/FOLH1/NAALADase I Antibody (NBP2-02045)(2)

Publications using NBP2-02045

• Rozolen JM, Teodoro TGW, Sobral RA Et al. Investigation of Prognostic Value of Claudin-5, PSMA, and Ki67 Expression in Canine Splenic Hemangiosarcoma Animals : an open access journal from MDPI 2021-08-14 [PMID: 34438863] (IHC-P)
• Millan C, Prause L, Vallmajo-Martin Q et al. Extracellular Vesicles from 3D Engineered Microtissues Harbor Disease-Related Cargo Absent in EVs from 2D Cultures Advanced healthcare materials 2021-04-22 [PMID: 33890421]

FAQs for PSMA/FOLH1/NAALADase I Antibody (NBP2-02045). (Showing 1 - 1 of 1 FAQs).

1. The spec sheet for this product does not indicate the type of antigen retrieval it will work well with. I have EDTA or citrate antigen retrieval. Will either one work just as well as the other?
   • Either of your antigen retrieval solutions should work well for this antibody. Our lab used 0.01M Sodium Citrate Buffer, pH 6.0. Below was their protocol: Antigen Retrieval - Immerse slides into staining dish containing Antigen Retrieval Solution. Place covered staining dish into the rice cooker. Add 120 mL d H2O and press "cook". When "cook" is turned to "warm" (about 20-30 min), unplug the cooker and remove the staining dish to the bench top. Allow to cool down, without cover, for 20 min. Here is a link to our Antigen Retrieval Protocol.

Bioinformatics

• Gene Symbol: FOLH1
• Entrez:
  • 2346(Human)