Human PSGL-1/CD162 ELISA Kit (Colorimetric)

ELISA: PSGL-1/CD162 ELISA Kit [NBP1-91256] - Standard curve

Product Discontinued

Human PSGL-1/CD162 ELISA Kit (Colorimetric) Summary

• Description: This kit is an enzyme-linked immunosorbent assay for quantitative detection.
• Specificity: The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human PSGL-1 positive serum. There was no crossreactivity detected.
• Standard Curve Range: 1.6 - 50 U/mL
• Sensitivity: 1.0 U/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 6.60%
• Intra-Assay: CV% < 3.20%
• Spike Recovery: The amount of endogenous human PSGL-1 in unspiked serum was subtracted from the spike values. The recovery ranged from 83% to 104% with an overall mean recovery of 94%.
• Sample Volume: 100 uL (1:20 prediluted)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SELPLG

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human PSGL-1/CD162 ELISA Kit (Colorimetric)

• CD162 antigen
• CD162
• P-selectin glycoprotein ligand 1
• PSGL1
• PSGL-1
• PSGL-1cutaneous lymphocyte-associated associated antigen
• selectin P ligandCLA
• SELPLG

Background

Selectins are part of the broader family of cell adhesion molecules. The family of selectins consists of three structurally and functionally related molecules, L-selectin, P-selectin and E-selectin. P-selectin glycoprotein ligand-1 is found on white blood cells and endothelial cells and plays an important role in the recruitment of white blood cells into inflamed tissue. PSGL-1 binds with the highest affinity to P-selectin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for PSGL-1/CD162 ELISA Kit (NBP1-91256). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SELPLG