PSGL-1/CD162 Antibody (TC2) - BSA Free

Flow (Cell Surface): PSGL-1/CD162 Antibody (TC2) [NBP1-44599] - Analysis using the PE conjugate of NBP1-44599. Surface staining of human peripheral blood cells with anti-human CD162 (clone TC2).

Product Discontinued

PSGL-1/CD162 Antibody (TC2) - BSA Free Summary

• Immunogen: Human thymocytes.
• Specificity: The antibody TC2 reacts with CD162, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes; it is also expressed on a subpopulation of B lymphocytes and CD34^+ bone marrow cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SELPLG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry 1.5-2.5 ug/ml
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PSGL-1/CD162 Antibody (TC2) - BSA Free

• CD162 antigen
• CD162
• P-selectin glycoprotein ligand 1
• PSGL1
• PSGL-1
• PSGL-1cutaneous lymphocyte-associated associated antigen
• selectin P ligandCLA
• SELPLG

Background

CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSGL-1/CD162 Antibody (NBP1-44599)(1)

Publication using NBP1-44599

• Wake H, Mori S, Liu K et al. Histidine-Rich Glycoprotein Prevents Septic Lethality through Regulation of Immunothrombosis and Inflammation. EBioMedicine. 2016-06-04 [PMID: 27333033] (Human)
  
  Details:
  This citation used the PE version of this antibody.

Bioinformatics

• Gene Symbol: SELPLG