PSGL-1/CD162 Antibody (HECA-452) [FITC]

Product Discontinued

PSGL-1/CD162 Antibody (HECA-452) [FITC] Summary

• Immunogen: The immunogen was the full length version of the protein: CLA (HECA-452)
• Specificity: CLA (HECA-452)
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: SELPLG
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry 5-20 ul/test (10^6 cells in 100 ul)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: The HECA-452 antibody cross-reacts with mouse skin homing lymphocytes. Purity is > 85%. IHC use reported in literature (see Duijvestijn AM, et al).
• Control:

  SR-BI Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2) and 0.2% (w/v) BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for PSGL-1/CD162 Antibody (HECA-452) [FITC]

• CD162 antigen
• CD162
• P-selectin glycoprotein ligand 1
• PSGL1
• PSGL-1
• PSGL-1cutaneous lymphocyte-associated associated antigen
• selectin P ligandCLA
• SELPLG

Background

Cutaneous Lymphocyte-assiciated Antigen (CD162 ) is a 140 kD homodimer protein recognized by a unique mAb HECA-452. It is expressed on T lymphocytes in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells, as well as on monocytes, granulocytes and activated endothelial cells. CD162 is a carbohydrate epitope of sialic acid- and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on majority of peripheral blood leukocytes. CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering and rolling. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA, and is suitable for staining formalin-fixed paraffin embedded tissue sections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78041)(3)

Publications using NB100-78041

• Duijvestijn AM, Horst E, Pals ST et al. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452. Am J Pathol. 1988-01-01 [PMID: 3276207] (IF/IHC)
• Berg EL, Yoshino T, Rott LS et al. The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1. J Exp Med. 1991-12-01 [PMID: 1720810] (B/N)
• Picker LJ, Kishimoto TK, Smith CW et al. ELAM-1 is an adhesion molecule for skin-homing T cells. Nature. 1991-02-01 [PMID: 1705666] (IF/IHC)

Bioinformatics

• Gene Symbol: SELPLG