PSGL-1/CD162 Antibody (HECA-452) [DyLight 680]

Product Discontinued

PSGL-1/CD162 Antibody (HECA-452) [DyLight 680] Summary

• Immunogen: Human tonsil stroma
• Specificity: Cutaneous Lymphocyte Antigen
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: SELPLG
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow (Cell Surface)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: The HECA-452 antibody cross-reacts with mouse skin homing lymphocytes. Purity is > 85%. IHC use reported in literature (see Duijvestijn AM, et al).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: IgM purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for PSGL-1/CD162 Antibody (HECA-452) [DyLight 680]

• CD162 antigen
• CD162
• P-selectin glycoprotein ligand 1
• PSGL1
• PSGL-1
• PSGL-1cutaneous lymphocyte-associated associated antigen
• selectin P ligandCLA
• SELPLG

Background

Cutaneous Lymphocyte Antigen (CLA) is a single-pass type I membrane homodimer protein (140kDa) that collects in normal and inflamed skin. It is expressed on T lymphocytes, subsets of peripheral blood memory T cells, Natural Killer cells, memory B cells and dendritic cells, as well as on monocytes and granulocytes. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1). CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in leukocyte tethering and rolling over vascular surfaces during inflammation. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA and is appropriate for staining formalin-fixed paraffin embedded tissue sections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SELPLG