PSGL-1/CD162 Antibody (3E2.25.5 (PL1))

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51736
    • Availability
      Product Discontinued

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PSGL-1/CD162 Antibody (3E2.25.5 (PL1)) Summary

Immunogen
Human neutrophils.
Specificity
Recognizes the human CD162 cell surface glycoprotein, a 120kD molecule also known as P-Selectin Glycoprotein Ligand I (PSGL-1). CD162 is the major counter receptor for P-selectin and is involved in neutrophil attachment and rolling to endothelium. In peripheral blood CD162 is expressed by monocytes, granulocytes, T cells and some B cells. MON 8071 is a blocking antibody, inhibiting PSGL-1 and P-selectin interaction. Immunogen: Human neutrophils.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SELPLG
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
The antibody is suitable for immunohistology on frozen sections, paraffin sections, Western Blotting and Fuctional Assays.This product requires antigen retrieval using heat pre-treatment for staining of paraffin sections. Recommended dilution for paraffin section is 1:200.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 mg purified material with PBS
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for PSGL-1/CD162 Antibody (3E2.25.5 (PL1))

  • CD162 antigen
  • CD162
  • P-selectin glycoprotein ligand 1
  • PSGL1
  • PSGL-1
  • PSGL-1cutaneous lymphocyte-associated associated antigen
  • selectin P ligandCLA
  • SELPLG

Background

Cutaneous Lymphocyte-assiciated Antigen (CLA) is a 140 kD homodimer protein recognized by a unique mAb HECA-452. It is expressed on T lymphocytes in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells, as well as on monocytes, granulocytes and activated endothelial cells. CLA is a carbohydrate epitope of sialic acid- and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on majority of peripheral blood leukocytes. CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering and rolling. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA, and is suitable for staining formalin-fixed paraffin embedded tissue sections. CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kDa, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SELPLG
Entrez