PSF2 Antibody - BSA Free

Western Blot: PSF2 Antibody [NBP2-33825] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-GINS2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: PSF2 Antibody [NBP2-33825] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: PSF2 Antibody [NBP2-33825] - Staining of human colon shows moderate nuclear positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PSF2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: ELVTIIPNFSLDKIYLIGGDLGPFNPGLPVEVPLWLAINLKQRQKCRLLPPEWMDVEKLEKMRDHERKEETFTPMPSPYYMELTKLLLNHASDNIPKADEIRTLVKDMWDTRIAKLRVSADSFVRQQEAHAKLDNLTLMEINTSG
• Predicted Species: Mouse (92%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GINS2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PSF2 Protein (NBP2-33825PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PSF2 Antibody - BSA Free

• DNA replication complex GINS protein PSF2
• GINS complex subunit 2 (Psf2 homolog)
• GINS complex subunit 2
• HSPC037
• PSF2Pfs2

Background

PSF2, also known as DNA replication complex GINS protein PSF2, is a 185 amino acid protein that is 21 kDa, belongs to the GINS2/PSF2 family, localizes in the nucleus, plays an essential role in the initiation of DNA replication and progression of DNA replication forks, and seems to bind preferentially to single-stranded DNA. This protein is being studied for its involvement in breast cancer. The protein has been also linked to the unwinding of DNA, S Phase, DNA strand elongation, synthesis of DNA, DNA replication, cell cycle, and cell cycle, mitotic pathways where it interacts with CHEK2, GINS4, FANCF, GTF2A1L, KPNA4, LMNB1, and other proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSF2 Antibody (NBP2-33825)(1)

Publication using NBP2-33825

• Li X, Liu Y, Zhou L et Al. Construction and Validation of a Mutation-Related Model in Papillary Renal Cell Carcinoma and Associated Immune Infiltration Kidney Blood Press Res 2024-06-11 [PMID: 38714185]

Bioinformatics

• Gene Symbol: GINS2
• Uniprot:
  • Q9Y248(Human)